摘要
以小鼠艾氏腹水癌细胞经超声破碎的提取液为材料,依次用DE52和P11层析柱进行离子线性洗脱,分别在KCl浓度为0.17~0.2mol/L和0.25~0.27mol/L处DNA引物酶被洗脱下来。用大肠杆菌大片段DNA多聚酶Ⅰ延长引物法检测DNA引物酶比活性为8753U/mg,酶总获得为22.1%。
DNA primase was extracted from mouse Ehrlich Ascites Carcinoma cells. The crude enzyme was loaded on DE 52 column and P 11 column sequentially. The primase active fraction was eluted at a KCl concentration of 0.17 mol/L to 0.2 mol/L on DE 52 column and 0.25 to 0.27 mol/L on P 11 column. DNA primase activity was assayed with E coli. DNA polymerase ⅠKlenow fragment extended primers. The specific activity of the enzyme was 8 753 U/mg protein. Different length of primers were synthesized by primase and detected with autoradiograph.
出处
《中山医科大学学报》
CSCD
1997年第4期279-281,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金