原位PCR技术检测石蜡包埋脑组织中人巨细胞病毒DNA

Detection of human cytomegalovirus DNA in brain tissues embedded in paraffin by in situ polymerase chain reaction

应用原位聚合酶链反应（ＩＳＰＣＲ）技术检测了２５例尸检畸形胎儿石蜡包埋脑组织中人巨细胞病毒（ＨＣＭＶ）ＤＮＡ，并与普通ＰＣＲ及原位杂交（ＩＳＨ）进行了比较。ＩＳＰＣＲ、ＰＣＲ及ＩＳＨ检测阳性率分别为４４％，３６％及２０％。与ＩＳＨ相比较，ＩＳＰＣＲ不仅检出阳性率高，而且信号强度增强。研究结果提示，ＩＳ－ＰＣＲ是诊断ＨＣＭＶ感染的快速、敏感、特异的实用方法。

The authors describe the detection of human cytomegalovirus DNA in paraffin embedded,formalin fixed tissues of brain in autoptic congenital malformation infants by in situ polymerase chain reaction(ISPCR),and compared with the method of PCR by extracted DNA and in situ hybridization (ISH).Of 25 total cases examined,there were 11(44%),9(36%) and 5(20%) positive specimens by ISPCR,PCR and ISH,respectively.Using conventional in situ hybridization,the sensitiveness of detectable HCMV DNA were lower.When the ISPCR assay was done,increased positive rate and numbers of cells detectable HCMV DNA,and the positive signal was much more intensive.It suggests that ISPCR is a more sensitive,rapid and effective method for detecting HCMV infection.