凋亡相关基因bax与Th蛋白在帕金森病大鼠黑质和纹状体细胞中的表达

Relationship between the expression of bax and Th in the substantia nigra and striatum and Parkinson′s disease in rats

目的:利用动物模型探讨bax和Th蛋白表达与帕金森病(PD)的关系。方法:60只SD大鼠随机分为6组,即对照组及PD组(PD模型3d、7d、14d、21d、28d组)。PD组均通过脑立体定向注入6-羟基多巴胺,均在处死前腹腔注入阿朴吗啡,观察30min大鼠旋转的次数,连续至第4周每分钟大于7次者为成功的PD模型。对照组立体定向注入抗坏血酸生理盐水。处死后制作石蜡切片,用免疫组化法检测bax、Th蛋白的阳性细胞数。结果:PD组纹状体和黑质神经细胞存在细胞凋亡,与对照组差异存在显著性,且bax蛋白表达在PD组大鼠纹状体和黑质细胞中3d组为最高,其后很快下降,但显著高于对照组。而Th蛋白表达在大鼠纹状体和黑质细胞中对照组为最高,在PD组很快下降。结论:6-羟基多巴胺能诱发大鼠黑质细胞凋亡,bax作为前凋亡蛋白在启动和诱导黑质细胞凋亡方面起着关键性的作用。

Objective To investigate the relationship between the expressions of bax and Th proteins and Parkinson＇s disease （PD） via the animal model. Methods Sixty SD rats were randomly divided into control group and groups with PD of 3, 7, 14, 21, and 28 days. The rat model of hemiparkinson was constructed by an infusion of 6- hydroxydopamine （6-OHDA） to the right substantia nigra of the midbrain and the control was administered saline solution. The expressions of bax and Th proteins in the sustantia nigra and striatum were detected by immunohistochemistry. Results The groups with PD differed significantly with the control group in that neuronal apoptosis in the sustantia nigra and striatum was present. The expression of bax proteins peaked in the group with PD of 3 days and then rapidly decreased but still higher than that in the control group. The level of Th protein expression was the highest in the control group and quickly declined in the groups with PD. Conclusions 6-OHDA can induce neuronal apoptosis in the substantia nigra in rats. Bax, a pro-apoptotic protein, plays a key role in initiating and inducing apoptosis in the substantia nigra.