摘要
目的通过体外试验研究异鼠李素对人胃癌SGC-7901细胞生长的抑制作用。方法将异鼠李素作用于人胃癌SGC-7901细胞,四甲基偶氮唑蓝(MTT)法测定IC50,细胞计数法和集落形成试验检测细胞生长抑制率,3H胸腺嘧啶核苷(3H-TdR)掺入试验测定对细胞DNA合成的影响,倒置显微镜下观察人胃癌SGC-7901细胞的形态变化。结果异鼠李素作用于人胃癌SGC-7901细胞后,细胞生长明显受到抑制。不同剂量的异鼠李素处理后的细胞变圆、变小、脱落,排列稀疏,部分细胞内出现大小不等的空泡。结论异鼠李素在体外能明显抑制人胃癌SGC-7901细胞增殖,且存在剂量-效应关系。
OBJECTIVE To investigate the effect of isorhamnetin on growth of human gastric carcinoma cells in vitro. METHODS The human gastric carcinoma SGC-7901 cells were treated with isorhamnetin. IC50 of isorhamnetin on SGC-7901 cells calculated by 3-（4,5- dimethylthiazole-2-yl）-2,5 diphenyl tetrazoliumbromide （MTT）assay. The rate of growth suppression on SGC-7901 cells was evaluated by cell growth curve and clone formation. The effect of isorhamnetin on DNA synthesis of human gastric carcinoma SGC-7901 cells was evaluated by ^3H-thymidine （^3H-TdR） incorporation. Cell morphology was observed by inverted microscope. RESULTS Isorhamnetin had significantly inhibitory effect on growth of human gastric carcinoma SGC-7901 cells.The morphological changes of human gastric carcinoma SGC-7901 cells were observed after treated with isorhamnetin for 24h, such as rounder, smaller, desquamation, sparse arrangement, vacuole of different size and etc. CONCLUSIONS Isorhamnetin can significantly inhibit proliferation of human gastric carcinoma SGC-7901 cells in dose-response manner in vitro.
出处
《中国初级卫生保健》
2008年第6期58-59,共2页
Chinese Primary Health Care
基金
黑龙江省卫生厅资助项目(2006-305)
关键词
异鼠李素
胃癌细胞
细胞生长
抑制作用
isorhamnetin, human gastric carcinoma SGC-7901 cell. cell growth, inhibitory effect
