小麦-黑麦异源多倍化中的微卫星序列变异

Variation of Microsatellite Sequence during Wheat-rye Allopolyploidization

[目的]探测异源多倍化过程中微卫星序列变异。[方法]利用150对小麦微卫星引物调查了小麦-黑麦双二倍体形成过程中微卫星序列的变异情况。[结果]与杂交F1植株及亲本植株相比,28对引物从双二倍体中扩增产物发生了变异,而其余引物从亲本、F1植株及双二倍体中扩增的带型相同。[结论]这表明常发生在二倍体生物中的微卫星序列变异现象在植物异源多倍化过程中也会发生,异源多倍化可能是促进微卫星进化的又一个不可忽视的动力。

[Objective] The research aimed to detect microsatellite sequnce variation during aUopolyploidization. [Method] One hundred and fifty wheat SSR markers Were used to investigate the variation of microsateUite sequence during wheat-rye allopolyploidization. [Result] Compared with parental wheat and F1 hybrids, the products amplified by 28 SSR markers from amphidiploids had changed. However, the bands amplified by the other 122 SSR markers from the amphidiploids, parental wheat and F1 hybrids were identical. [Conclusion] Allopolyploidization might be an important factor induced microsatellite evolution.