固相萃取-HPLC法同时测定果蔬中多菌灵和噻菌灵残留量

Simultaneous Determination of Thiabendazole and Carbendazim Residues in Fruits and Vegetables by Solid Phase Extraction and HPLC

研究了一种可同时测定果蔬中噻菌灵和多菌灵残留量的固相萃取-高效液相色谱法。样品经乙酸乙酯提取,离心,混合相固相萃取小柱净化后,用配有二级管阵列检测器(PAD)的液相色谱仪检测,外标法定量。同时,以多菌灵和噻菌灵标准品进行添加回收率测定,结果显示方法对多菌灵的测定低限为0.025mg/kg,回收率为71.3%～92.5%;对噻菌灵的测定低限为0.025mg/kg,回收率为70.7%～84.2%;测定的相对标准偏差均不大于5.3%。该方法能满足果蔬中多菌灵和噻菌灵残留量常规检测的需要。

A solid phase extraction-high performance liquid chromatographic（HPLC） method was developed for the simultaneous analysis of carbendazim and thiabendazole in fruits and vegetables. Fruits and vegetables samples were extracted with ethyl acetate, centrifuged and cleaned up with mixed-mode SPE, and carbendazim and thiabendazole residues were determined by HPLC with PAD using external standard method. The determination limit was 0.025 mg/kg for both. The recoveries were 71.3-92.5% for carbendazim and 70.7-84.2% for thiabendazole, and the relative standard deviations were all no more than 5.3%. The method meets analysis requirements of the determination of carbendazim and thiabendazole in fruits and vegetables.