水稻中醚磺隆的残留分析方法

Determination Method of Cinosulfuron Residue in Rice

研究了水稻中醚磺隆的残留分析方法。样品经甲醇提取,石油醚、二氯甲烷转换萃取,C18小柱净化,高效液相色谱仪分离、紫外250nm检测。方法的回收率为69%～95%,变异系数为3.5%～9.0%。醚磺隆的最小检知量为2.0×10-9g,对田水、土壤、稻杆、谷壳、稻米的最低检出质量浓度和质量分数分别为0.005mg/L和0.01、0.05、0.05、0.01mg/kg。该方法样品适应性强,可用于环境和植物样本的醚磺隆残留检测。

A determination method of cinosulfuron residue was established. The residue was extracted by methanol, cleaned with liquid-liquid extraction and C18 SPE column. The target ingredient was separated by high performance liquid chromatography and detected by UV detector at 250 nm. The recoveries of the method ranged from 69 to 95%, with the coefficient of variation from 3.5 to 9.0%. The minimum detectable quantity of cinosulfuron standard was 2.0×10^-9g. The lowest detectable concentration of field water, soil, rice straw, rice shell and hulled rice samples were 0.005 mg/L, 0.01, 0.05, 0.05, 0.01 mg/kg, respectively. The determination method can be widely applied on different environmental and plant samples.