摘要
目的:探讨左金丸的主要成分吴茱萸碱和小檗碱对结肠癌HT29细胞凋亡及端粒酶催化亚基(hTERT)表达的影响,为临床应用左金丸治疗大肠癌提供实验依据。方法:体外培养人结肠癌HT29细胞,加入不同浓度的吴茱萸碱及盐酸小檗碱进行培养,采用活细胞计数试剂盒(CCK-8)检测细胞增殖,Annexin Ⅴ-FITC/PI双染色流式细胞术检测细胞凋亡变化,RT-PCR法检测hTERT的表达。结果:吴茱萸碱和盐酸小檗碱作用HT29细胞后,增殖明显受到抑制,呈剂量和时间依赖性;流式细胞仪检测表明吴茱萸碱和盐酸小檗碱能够诱导细胞凋亡,呈剂量依赖性;并且端粒酶hTERT表达降低,具有剂量依赖关系。结论:吴茱萸碱和盐酸小檗碱对结肠癌HT29细胞具有显著生长抑制作用,该抑制作用可能通过下调端粒酶hTERT基因表达,降低端粒酶的活性,从而诱导细胞凋亡。
Objective： To study the effects of evodiamine and berberine hydrochloride on cell apoptgsis and expression of hTERT-mRNA of the cultured human colorectal cancer cell line HT-29. Methods： HT-29 cells were treated with evodiamine and berberine hydrochloride at different concentrations. The proliferative status of HT-29 cells were measured by WST-8 assay. Apoptosis was detected by Annexin V-FITC/PI assay and hTERTmRNA expression was examined by RT-PCR. Results： Evodiamine and berberine hydrochloride displayed a-significant growth inhibitory effect on HT29 cell line in the dose and time-dependent manner . The flow cytometry analysis showed evodiamine and berberine hydrochloride could induced the apoptosis of HT29 cells, which showed significant dose-effect relationship. And the expression of hTERT- mRNA was decreased in concentration-dependent manner. Conclusion： Evodiamine and berberine hydrochloride can inhibit the proliferation of colorectal cancer cell line HT-29 and induced cell apoptosis, whose mechanisms may be related to down-regulation of hTERT gene expression and then decreased telomerase activity.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2008年第2期6-8,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(30400602)
