地黄寡糖对血管性痴呆大鼠学习记忆能力及海马乙酰胆碱的影响

The Effect of Rehmannia glutinosa oligosaccharides on learning and memory abilities and cerebral ACh in rat with vascular dementia

目的:探讨地黄寡糖对血管性痴呆大鼠学习记忆能力及海马乙酰胆碱的影响。方法:采用腹腔注射硝普钠后夹闭双侧颈总动脉10min,再灌10min,共两次的方式制备VD模型;用水迷宫对大鼠进行学习记忆能力测定;碱性羟胺比色法测定海马乙酰胆碱含量。结果:模型组大鼠与对照组相比学习记忆能力明显下降;与模型组相比,假手术组和地黄寡糖组的海马组织乙酰胆碱含量显著增多,且不同剂量地黄寡糖组之间具有剂量和效应关系。结论:地黄寡糖可以改善VD大鼠学习记忆能力,该作用可能与提高海马乙酰胆碱含量有关。

Objective： to observe the effect of Rehmannia glutinosa oligosaccharides （ROS） on learning and memory abilities and ACh in rat hippocampus with vascular dementia （VD）. Methods： Rat model of VD was established by repeated ischemia/reperfusion and pretreated with sodium nitroprusside; the learning and memory abilities were tested by water maze; the content of Ach was determined by alkaline hydroxylamine. Results： Compared with the sham operation group, the model group＇s learning and memory ability was significantly decreased, Compared with the model group, the rats of the sham - operation group and ROS group showed the content of Ach in hippoaampus were significantly increased. ROS significantly improved the learning and memory abilities in rat with VD, and the contents of ACh were increased. Conclusion ： ROS can improve the spatial memory of rat which may be related to its effect of increasing ACh in hippocampus.