注射用苦碟子对心肌缺血再灌注损伤大鼠心肌酶和血黏度的影响

Effect of Ixeris sonchifolia extracts (KDZ) for injection on myocardial enzymes and blood viscosities of myocardial ischemia reperfusion injury rats

目的:研究注射用苦碟子(Ixeris sonchifolia extracts for injection,KDZ)对心肌缺血再灌注损伤大鼠心肌梗死面积、心肌酶和血黏度的影响。方法:大鼠以KDZ(2.5、5.0、10g生药/kg·d,ip),或0.9%NaCl治疗2天后,结扎大鼠冠状动脉左前降支60min,再灌注120min造成心肌缺血再灌注模型,然后再立即给药一次。计算心肌梗死面积(MIS),测定血清肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)活性及低、中、高切变率下全血黏度和血浆黏度。结果:与再灌注模型组比较,KDZ中、大剂量能明显缩小MIS,显著降低血清CPK、LDH及AST活性,小剂量也能降低血清LDH活性;KDZ大剂量使全血低、中、高切黏度及血浆黏度明显降低,中剂量也能降低全血低切黏度。结论:KDZ可缩小心肌缺血再灌注损伤大鼠的MIS,降低血清CPK、LDH、AST活性及血液黏度,发挥保护作用。

Objective：To study the protective effect of Ixeris sonchifolia extracts （KDZ） for injection on myocardial ischemia reperfusion injury in rats. Methods：Mter 2 days treatment with KDZ （2.5,5.0 and 10g crude drug/kg · d,ip ） or 0.9% NaCl in rats,the myocardial ischemiareperfusion model was induced by occulusion of left anterior descending coronary for 60min and reperfusion for 120min, followed by giving KDZ or 0.9% NaCl once again. Then the myocardial infarct size（MIS） was detected; the activities of creatine phosphokinase （CPK）, aspartate aminotransferase （AST） , and lactate dehydrogenase （LDH） in blood serum were examined, and the high,medial, and low shearing blood viscosities and plasma viscosity were determined. Results：compared with that of the model control, the MIS and the activities of CPK, AST, LDH were significantly reduced （ P 〈 0.05 or P 〈 0.01 ）, and viscosities of blood and plasma were declined （ P 〈 0.05 or P 〈 0.01 ） in rats treated by KDZ. Conclusion：KDZ has protective effect on myocardial ischemia reperfusion injury in rats.