咳喘宁对支气管哮喘大鼠肺组织IFN-γ和IL-4含量的影响

Effect of Kechuanning (咳喘宁) on the contents of IFN-γ and IL-4 in lung tissue of bronchial asthmatic rats

目的:观察咳喘宁对支气管哮喘大鼠肺组织干扰素γ(IFN-γ)、白介素4(IL-4)含量的影响。方法:以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4周后处死大鼠,观察各组大鼠肺组织IFN-γ和IL-4含量和病理组织学变化。结果:与正常组比较,模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织IL-4含量明显增加,IFN-γ和IL-4比值明显降低,IFN-γ含量有所升高,但无显著性差异;与模型组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织IL-4含量,升高IFN-γ含量以及IFN-γ和IL-4比值;且咳喘宁高剂量组优于桂龙咳喘宁组。结论:咳喘宁可通过调节Th1/Th2细胞因子之间的平衡,抑制气道炎症。

Objective： To observe effect of Kechuanning on the contents of interferon-γ（IFN-γ） and interleukin 4 （IL-4） in lung tissue of bronchial asthmatic rats. Methods： 40 rats were randomly divided into 5 groups： the normal group, the model group, Kechuanning high-dose group （27g/kg）, Kechuanning low-dose group（13.5g/kg） , Guilongkechuanning control group（0.45g/kg） ,8 rats each group. The bronchial asthmatic model was established by egg protein sensibilization and long-term inhalation provocation. The rats of each treatment groups were lavage administration each day from the first time of provocation to execution. After 4 weeks of treatment, the rats were killed and lung tissue were taken to dyeing of HE to be observed. The contents of IFN-γ,and IL-4 in lung tissue were determined. Result： Compared with the normal group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, and the content of IL-4 in lung tissue were increased and the ratio of IFN-γ / IL-4 were decreased in the model group（ P 〈0.01 ） , the content of IFN- γ,were increased, but not remarketable different（ P 〉 0.01 ） ; Compared with the model group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, the content of IL-4 in lung tissue were decreased and the content of IFN-γand the ratio of IFN-γ / IL-4 were increased in each treatment group （ P 〈 0.05 or P 〈 0.01 ）, furthermore, the effect of high-dose Kechuanning group were super to that of Guilongekechuanning group （ P 〈 0.05）. Conclusion： The Kechuanning could regulate the banlance of cytokines Thl and Th2 to restrain air passage inflammation.