川芎嗪和阿苯达唑联合治疗小鼠继发性泡球蚴病的疗效观察

Effect of tetramethylpyrazine combined with albendazole on the secondary Alveolar echinococcosis in mice

目的通过观察中药川芎嗪和阿苯达唑单用及联合使用对小鼠继发性泡球蚴病的疗效,探讨中西药结合治疗泡球蚴病的新途径。方法应用川芎嗪和阿苯达唑对小鼠继发性泡球蚴病治疗60d后,检测小鼠泡球蚴湿重、脾脏指数、抑囊率、血清TNF-α和SOD含量以及肝组织匀浆中NO和NOS的含量,并对泡球蚴组织进行病理组织学和超微结构观察。结果川芎嗪和阿苯达唑单用及联合使用对小鼠泡球蚴的抑制率分别为66.71%、85.59%和95.44%,差异有统计学意义(χ2=28.520,P<0.05);脾脏指数、血清TNF-α含量、肝脏NO、NOS含量与模型组比较差异有统计学意义(F=8.614,P<0.05);血清SOD含量川芎嗪组和川芎嗪+阿苯达唑组与模型对照组比较差异有统计学意义(F=7.359,P<0.05)。结论1)川芎嗪和阿苯达唑对小鼠泡球蚴的生长均有明显抑制作用;2种药物联合使用具有协同治疗效果;2)川芎嗪能显著改善阿苯达唑对小鼠肝功能的损伤。

Objective To explore co-administration effect of tetramethylpyrazine with albendazole（ABZ） on Alveolar echinococcosis（AE） in mice. Methods The mice were divided into five groups, including single tetramethylpyrazine, single albendazole, combination of the two, control and blank. After being treated 60 days, the mice were dissected. The major judging criteria included cyst wet weight of AE and spleen index, serum TNF-a and SOD level in experimental mice. NO content and NOS activity in hepatic homogenate from the AE animal model treated by drugs also were measured by chromatometry, histopathological changes of cyst both microscopically electron-microscopically. Results The inhibition rates of tetramethylpyrazine, albendazole and co-administration against growing of Echinococcus multilocularis cyst were 66.71%, 85.59% and 95.44%, respectively. The thymus index of control group was lower than that of therapeutics groups, but the spleen index was higher than that of therapeutics groups （P〈0.05）. The serum TNF-α of therapeutics groups were lower than that of control group （P〈0.05）. The level of SOD in serum increased （F= 7. 359, P〈 0. 05）. While in hepatic tissue homogenates, the NO content and NOS activity decreased in all groups of the treated mice （P〈0. 05）. Tetramethylpyrazine against E. multilocularis had obviously inhibitory effect. Conclusion Tetrameth-ylpyrazine could enhance immune of mice. The combination with albendazole had stronger inhibition action. These results indicated that tetramethylpyrazine could strengthen albendazole＇s effect and had synergia action with albendazole against E. multilocularis, and tetramethylpyrazine could significantly improve hepatic functions of the mouse infected with E. multilocularis.