银杏酮酯对衰老模型大鼠学习记忆及海马细胞因子的影响

Effects of Ginkgo Biloba Extract on Learning and Memory and Cytokines of Hippocampus in Senile Rats

目的:观察银杏酮酯(GBE50)对D-半乳糖致衰老大鼠学习记忆障碍及海马细胞因子含量的影响,探讨银杏提取物改善学习记忆障碍的机制。方法:将SD大鼠随机分成3组:正常组、模型组和银杏酮酯组。采用腹腔注射D-半乳糖的方法建立衰老大鼠模型;GBE50组在造模第21天开始按150mg/kg给予GBE50灌胃,每天1次,持续21d;正常组和模型组灌等量的含1%CMC-Na的蒸馏水。治疗结束后采用Morris水迷宫观察大鼠行为学变化以及放射免疫分析方法检测各组大鼠海马IL-1β、IL-6和TNF-α的含量。结果:与正常组比较,模型组逃避潜伏期明显延长,距离百分比明显减少(P<0.05,P<0.01);而与模型组比较,GBE50组逃避潜伏期显著缩短,距离百分比明显增大(P<0.05,P<0.01)。与正常组比较,模型组大鼠海马IL-1β和TNF-α含量显著增高,IL-6含量明显降低(P<0.05,P<0.01);而与模型组比较,GBE50组大鼠海马IL-1β含量明显降低,IL-6含量显著升高(P<0.05,P<0.01)。结论:GBE50对由D-半乳糖所致衰老大鼠学习记忆障碍具有改善作用,同时对海马IL-1β、IL-6含量均有不同程度的调节作用,GBE50改善记忆障碍作用可能与抑制D-半乳糖引发的中枢神经系统免疫炎性反应有关。

Objective： To observe the effects of Ginkgo biloba extract （GBE50） on learning and memory impairment induced by D-galactose and contents of IL-1β, IL-6 and TNF-et in hippocampus of senile rats, in an attempt to study its mechanisms in improving learning and memory. Methods： SD rats were randomly divided into 3 groups： con- trol group, model group and treatment group. In modeling, D-galactose was injected into abdomen according to 100mg/ kg in the morning for 42 days. From the 21st day after modeling, GBE50 was given through gastric perfusion accord- ing to 150mg/kg in treatment group every day, and continuously for 21 days. Distilled water containing 1% CMC-Na was given in control and model groups. After treatment, Morris water maze tests were used to detect the change of rat behavior and the contents of IL-1β, 1L-6 and TNF-et in hippocampus in all groups by radioimmunoassay. Results： The escape latency of the rats in model group was significantly longer than that in control group; the percentage of distance was lower （P 〈 0.05, P 〈 0.01）; however, the escape latency of the rats in treatment group was significantly shorter than that in model group, and the percentage of distance was higher （P 〈 0.05, P 〈 0.01）. The contents of IL-1β and TNF-et in hip- pocampus of model group were significantly higher than those of control group, but contents of IL-6 were lower （P 〈 0.05, P 〈 0.01）. Compared with model group, the contents of IL-1β in treatment group were significantly decreased; and the contents of IL- 6 were significantly increased （P 〈 0.05, P 〈 0.01）. Conclusion： GBE50 can improve the impaired learning and memory of senile rats induced by D-galactose by regulating the contents of IL-1β and IL-6 in hippocampus, and inhibiting immune in- flanunatory reaction in central nerve system induced by D-galactose.