添加有机酸对Clostridium acetobutylicum合成丙酮和丁醇的影响

Effect of acetic and butyric acids on fermentation production of acetone-butanol by Clostridium acetobutylicum

为提高丙酮-丁醇梭菌厌氧发酵生产丙酮和丁醇的能力,在发酵过程中添加有机酸(乙酸和丁酸),考察其对菌体生长、溶剂合成影响。实验表明:当添加1.5 g/L乙酸时能够促进菌体的生长,促进丙酮的合成,在600 nm处的最大OD值比参照值高出18.4%,丙酮的最终质量分数提高了21.05%,但不能促进丁醇的合成;当添加1.0g/L丁酸时能够促进菌体生长,促进丁醇的合成,在600 nm处的最大OD比参照值高22.29%,丁醇的最终质量分数比对照组提高了24.32%,但不能促进丙酮的合成。

To improve acetone-butanol production efficiency by Clostridium acetobutylicum, acetic and butyric acid were added to the fermentation broth to investigate the effect on cell growth and production of acetone-butanoL Addition of 1.5 g/L acetic acid increased the cell growth and enhanced acetone production, the final concentration of acetone was 21.05%, and the butanol production was not improved. Addition of 1.0 g/L butyric acid increased the cell growth and enhanced butanol production, the final concentration of butanol was 24. 32%, while the acetone production was not improved.