摘要
目的建立丹参缓释片中丹参素的含量测定方法。方法采用RP-HPLC法,色谱柱为Eurospher-100C18（4.6mm×250mm;5μm）;流动相：甲醇-1%冰醋酸（15∶85）;流速：1.0ml/min;紫外检测波长280nm;柱温：室温。结果丹参素的线性范围为9.055-144.879μg/ml;相关系数为0.9999;加样平均回收率为97.34%（RSD=0.80%）。结论该法操作简单,结果准确,重复性好,可作为该制剂的质量控制方法。
Objective： To develop a method for the quantitative determination of danshensu in sustained release tables of salvia miltiorrhiza. Method： By RP HPLC the chromatographic column is Eurospher - 100 C18 （46mm ×250mm, 5μm）, the mobile phase is methanol 1% acetic acid （15： 85）, the flow rate is set 10ml/min; the detection wavelength is at 280nm. Results： The linearity ranges of Danshensu are 9. 055 - 144879μg/ml, the correlation is 0. 9999; the average recovery of adding sample is 97. 34% （ RSD =0. 80% ） . Conclusions： The operation is simple, the result is accurate, the reproducible character is good. It is for the quality control of sustained release tables of salvia miltiorrhiza.
出处
《湖北中医学院学报》
2008年第1期34-36,共3页
Journal of Hubei College of Traditional Chinese Medicine
基金
湖北省教育厅科学技术研究项目
[D200716005]
关键词
丹参素
提取
反相高效液相色谱法
丹参缓释片
Danshensu
extraction
RP HPLC
sustained release tables of salvia miltiorrhiza
