刚果12号桉胚乳愈伤组织诱导和三倍体植株再生研究

Callus Induction and Plant Regeneration from Endosperm Culture of Eucalyptus 12ABL

以刚果12号桉授粉13-16d的胚珠为外植体，消毒处理后对胚珠进行离体培养。结果表明：最佳诱导愈伤组织的培养基为MS＋1．5mg·L^-1 6-BA＋1．0mg·L^-1 IAA，诱导频率32．5％；将红色紧密的愈伤组织转至MS＋1．0mg·L^-1 6-BA＋1．0mg·L^-1 NAA的培养基上，经连续继代后形成不定芽，根据多倍体的形态特征，对再生植株进行分离培养，生根后取根尖染色体显微观察，染色体数目均为33。

Taking ovules pollinated 13- 16 d of eucalyptus 12ABL as materials, the endosperm development was at vigorous stage, suitable for culture in vitro at this moment. The experiments showed that endosperm inoculated on MS medium supplemented with 1.5 mg · L^-1 6-BA and 1.0 mg·L^-1 IAA, gave the highest callus induction rate （32.5 %）. Endesperm callus cultured in medium MS containing 1.0 mgL^-1 6- BA and 1.0 mg · L^-1 , could regenerate more shoots than those in any other media. Many endosperm-derived plantlets were obtained and were proved to be triploid plants by observing the chromosomes under microscope.