柴松胚培养诱导芽形成的初步研究

A Preliminary Study on Inducing Buds of Pinus tabulaeformis f.shekannesis

为了对柴松组织培养进行系统研究，分析了柴松外植体处理方法和不同6-BA浓度对胚培养诱导等形成的影响，结果表明：适宜于柴松芽诱导的培养基为1／2MS＋6-BA 2．0mg·L^-1；经低温沙藏的柴松成熟种胚生长良好，外植体用0．1％HgCl。最佳消毒时间为5．0-5．5min；外植体的最佳放置方式为水平放置。

1/2 MS was used as the basic medium for bud proliferation of Pinus tabulaeforrnis f. shekannesis. The best concentration effective of 6-BA was 2. 0 mg · L^-1. The results of selecting the optimal explants showed that the mature embryo which was preserved at low-temperature in sands is the best explants, the suitable disinfectant time in 0.1% HgCl2 for 5.0-5.5 min, the level location of explants was the best way.