摘要
为了对柴松组织培养进行系统研究,分析了柴松外植体处理方法和不同6-BA浓度对胚培养诱导等形成的影响,结果表明:适宜于柴松芽诱导的培养基为1/2MS+6-BA 2.0mg·L^-1;经低温沙藏的柴松成熟种胚生长良好,外植体用0.1%HgCl。最佳消毒时间为5.0-5.5min;外植体的最佳放置方式为水平放置。
1/2 MS was used as the basic medium for bud proliferation of Pinus tabulaeforrnis f. shekannesis. The best concentration effective of 6-BA was 2. 0 mg · L^-1. The results of selecting the optimal explants showed that the mature embryo which was preserved at low-temperature in sands is the best explants, the suitable disinfectant time in 0.1% HgCl2 for 5.0-5.5 min, the level location of explants was the best way.
出处
《西北林学院学报》
CSCD
北大核心
2008年第3期114-116,共3页
Journal of Northwest Forestry University
基金
国家科技部科技成果重点推广项目(2001EC0003222)
关键词
柴松
芽诱导
外植体
培养基
Pinus tabulaeforrnis f. shekannesis
inducing adventitious buds
explant
selecting of medium