花椒组织培养再生体系的建立

Establishment of Tissue Culture Regeneration System of Zanthoxylum bungeanum

以凤椒的嫩茎和嫩叶为外植体,研究了不同种类和浓度的细胞分裂素(TDZ、ZT、6-BA和KT)和生长素(IBA和NAA)组合对花椒组织培养再生体系的影响,建立了完整的花椒组培再生体系。结果表明:嫩叶是诱导花椒愈伤组织较好的材料;MS+2,4-D 0.5 mg.L-1+BA 0.5 mg.L-1能成功诱导嫩叶产生愈伤组织,诱导率达90%,且愈伤组织生长良好;MS+TDZ 0.03 mg.L-1+BA 0.1 mg.L-1能成功的诱导愈伤组织分化出正常的不定芽,诱导率达70%;芽增殖的适宜培养基为MS+0.4 mg.L-16-BA+0.3 mg.L-1IBA,增殖系数为20;1/4MS+0.4 mg.L-1IBA能成功诱导健壮的无根苗生根,生根率90%以上。

Soft stems and amture leaves were used as explants to study the tissue culture regeneration system of Zanthoxylum bungeanum. The results showed that the amture leaves were suitable materials for calli inducing and the best medium was MS＋0.5 mg·L^-1 2,4-D＋0.5 mg·L^-1 BA,and the inducing ratio was as high as 90%. The medium of MS＋0.03 mg·L^-1 TDZ＋0.1 mg ·L^-1 NAA was the best one for shoot regeneration from calli. The regeneration ratio could be up to 70%. On the medium of MS＋0.4 mg ·L^-1 6-BA＋0.3 mg ·L^-1 IBA, the adventitious could be multiplied rapidly. The multiplication coefficient was about 20. When the shoots with a length of 2era were cultured on the medium of 1/4MS＋0.4 mg·L^-1 IBA, more than 90% of them rooted and developed normally.