摘要
水稻种子在极低温(-196℃)条件下进行(137)~Csγ射线辐照,然后进行热冲击(65℃)后处理。结果表明,极低温加热冲击处理的辐射防护效应大于极低温处理。在10—50krad剂量范围内,水稻主要性状的突变率,极低温加热冲击组及热冲击组大于常温组,而常温组又大于极低温组;在70—90krad范围内,极低温加热冲击组大于极低温组,其余两组全部死亡。各处理组在最适宜诱变剂量下的突变频率:极低温加热冲击组最高,常温组最低,其余两组居中。
Dormant seeds of rice (Oryza sativa L.) variety Erjiufeng were irradiated with various dose of 137Cs gamma rays at-196℃ and immediately followed by soaking in hot water (65℃) for 2 minutes. The damage on vitality index, seedling height, seedling survival rate, seed-setting rate and micronucleus rate in the MI generation was found to be normal temperature group > heat shock group > extremely low temperature group > extremely low temperature and heat shock group; and the total mutation frequency in the M2 generation was: extremely low temperature and heat shock group > heat shock group > normal temperature group > extremely low temperature group. It is indicated from the experimental results that the extremely low temperature and heat shock treatment is effective for enhancing mutation frequency.
出处
《核农学报》
CAS
CSCD
1990年第3期129-134,共6页
Journal of Nuclear Agricultural Sciences
关键词
水稻
种子处理
^137Csγ射线
诱变
Rice, 137Cs γ-ray,extremely low temperature, heat shock,mutagenic efficiency
