中华猕猴桃耐盐变异体筛选

SELECTION OF SALT-TOLERANT VARIANTS FROM Actinidia Chinensis

取中华猕猴桃(Actinidia chinensis)101品系试管苗叶片为外植体,诱导愈伤组织,将愈伤组织移入含NaCl的MS培养基选择8次,培养基内NaCl的浓度逐步增加,γ射线作为诱变剂,已筛选出耐0.5％、0.7％、1.0％NaCl的变异细胞系,并均再生出完整植株。试验结果表明:1.MS+0.5ppm 2,4-D+1.0ppm Zt是最佳的脱分化培养基;MS+1.0ppm Zt+0.2ppm IBA是最佳的分化培养基;生根培养基可采用MS+0.5ppm IBA。2.0.5％NaCl可作为耐盐筛选的盐分临界值。3.γ射线处理对愈伤组织的生长及细胞的大小均有显著的影响,5kR可作为变异体筛选的诱变照射量。

The leaves of Actinidia chinensis in vitro were used as explants in tissue culture to induce calli.The calli were cultured in MS medium containing NaCl for eight passages.The. concentration of NaCl in the medium was increased step by step. 60Coγ-rays were used as mutagens. The experiment for selecting medium was designed with orthogonal method. The cell lines tolerated to 0.5, 0.7,1.0% NaCl were established separately. The regenerated plants from various NaCl tolerant cell lines were obtained. The results are as follows.1.MS + 0.5 ppm 2,4-D+l.0 ppm Zt was the best medium for inducing callus,MS + 0.2ppm IBA+1.0ppm Zt was the best for differentiation and MS + 0.5ppm IBA was the best for rooting.2.Growing of the callus was inhibited by NaCl.The growth of callus decreased significantly in 0.3% NaCl, and decreased very significantly in 0.5% NaCl.Therefore, 0.5% NaCl concentration can be considered as the critical value for salt-tolerance selection.3.γ-rays over 5kR not only caused increase of the cell size but also inhibited growth of the callus.So, 5kR γ-rays can be considered as optimum mutation dose.