摘要
为提高硝化菌的亚硝酸盐氧化能力,利用统计试验设计(Plackett-Burman和Box-Behnken设计)优化得到一最佳培养基:NaHCO32.0g·L-1;NaNO22.36g·L-1;Na2CO30.37g·L-1;NaCl0.34g·L-1;KH2PO40.05g·L-1;MgSO4·7H2O0.05g·L-1;FeSO.47H2O0.03g·L-1。在此条件下,硝化菌的最大亚硝酸盐氧化速率达到905.0mgNO2-N·(gMLSS.d)-1(mixed liquor suspended solids,MLSS,混合液悬浮固体)。将50L降解速率为850mgNO2-N·(gMLSS,d)-1的硝化菌(浓度为1.99gVSS·L-1)(volatile solid,VSS,挥发性固体)投加至0.6hm2的养殖水体中,7d内试验水体中的亚硝酸盐浓度即降至安全浓度以下。
The statistical experimental design （Plackett-Burman and Box-Behnken design） was applied to optimize the culture medium of nitrite oxidizing bacteria for improving the nitrite oxidizing rate. Estimated optimum medium composition of the nitrite oxidizing rate was as follows： NaHCO3 2.0 g·L^-1 ; NaNO2 2. 36 g·L^-1 ; Na2CO3 0. 37 g·L^-1 ; NaC1 0. 34 g·L^-1 ; KH2PO4 0. 05 g.he nitrite oxidizing rate reached a maximum at 905.0 mg NO2-N. （ g MLSS.d）^-1 （ mixed liquor suspended solids, MLSS）. In the field trial, 50 L of nitrite oxidizing bacteria concentrate （1.99 g VSS·L^-1 ） （volatile solid, VSS） with 850 mg NO2-N·（g MLSS.d）^ -1 were added to 0. 6 hectares of the aquaculture water. Nitrite level in all treated ponds remained very low compared to the steady increase observed in all of the control ponds during 7 days.
出处
《南方水产》
2008年第3期7-14,共8页
South China Fisheries Science
