膀胱移行细胞癌中PTEN和p53基因的表达及临床意义

Expression and Significance of PTEN and P53 Gene in Bladder Transitional Cell Cancer

【目的】研究第10号染色体丢失的张力蛋白同源的磷酸酶基因(PTEN)及p53在膀胱移行上皮细胞癌(BTCC)组织中的表达水平及其与BTCC生物学行为的关系及临床意义。【方法】以免疫组化SP法检测10例正常膀胱黏膜及69例BTCC组织中的PTEN及p53基因的表达水平。【结果】在10例正常膀胱组织中PTEN基因均表达阳性或强阳性,p53基因有1例弱阳性表达,其余9例表达阴性。在69例BTCC组织中PTEN高表达组为46例(66.7%),其中阳性15例,强阳性31例,p53阳性高表达组为39例(56.5%),其中阳性22例,强阳性17例。随着肿瘤分期、分级的增加PTEN表达显著减少(P<0.01);而p53的表达显著增强(P<0.01);PTEN的表达随肿瘤分期、分级而减少与p53的表达增强呈显著负相关(P<0.01)。【结论】PTEN与p53可能成为估计BTCC恶性程度及肿瘤侵袭性的一项有用的指标,p53基因与PTEN基因的表达可能起互相调节作用。

[Objective]To investigate the protein expression of the tumor suppressor gene PTEN（phosphatase and tensin homology deleted on chromosome ten） and P53 gene and their possible roles in the development and progression of bladder transitional cell carcinoma （BTCC）. [Methods] Immunohistochemical method was used in detecting PTEN and P53 in 69 BTCC and 10 normal bladder tissues. [Results] The expression of PTEN was positive or strong positive in 10 normal bladder tissues. The expression of P53 gene was weakly positve in one normal bladder tissue and nagative in 9. Forty-six BTCC tissues were high expression of PTEN, 15 positve and 31 strong positive. Thirty-nine BTCC tissues were high expression of P53, 22 positive and 17 strong positive. The positive expression rate of PTEN and P53 gene in BTCC were 66. 7% and 56.5%respectively. The expression of PTEN significantly decreased and the expression of P53 increased with the increment of pathological grades（ P〈0. 001）. There was significantly inverse correlation between PTEN and P53 expression（ P 〈0. 0001）. [Conclusion] The abnormal expressions of PTEN and P53 gene rnay play an important role in the malignant progression of BTCC . The expression of PTEN and P53 gene may be regulated by each other.