摘要
β-1,3-1,4-葡聚糖酶活性检测结果表明,从辣椒根际筛选的拮抗菌枯草芽孢杆菌(Bacillus subtilis)SC2-4-1能产生β-1,3-1,4-葡聚糖酶。以菌株SC2-4-1的基因组DNA为模板,用PCR方法克隆了该菌的葡聚糖酶基因gluB,其开放阅读框为711bp,编码237个氨基酸。Blast分析,该序列与已报道的多粘类芽孢杆菌(Paenibacillus polymyxa)ATCC 842的β-1,3-1,4-葡聚糖酶基因gluB相似性为85%。所得基因序列的系统发育分析显示,该基因属于β-1,3-1,4-葡聚糖酶基因。DNAMAN软件比对,所得葡聚糖酶氨基酸序列具有催化裂解β-1,3-和β-1,4-糖苷键的葡聚糖酶活性位点。
Detection of β-1,3-1,4- glucanase activity reveals that antagonistic bacterium Bacillus subtilis SC2-4-1 screened from the rhizosphere of capsicum can produce β-1,3-1,4-glucanase. Glucanase gene gluB of the strain was cloned by PCR. with genomic DNA of B. subtilis SC2-4-1 as template. Its open reading frame is 711bp coding 237 amino acids. Blast analysis reveals that glucanase gene sequence is identical to β-1,3-1,4-glucanase gene gluB reported from Paenibacillus polymyxa ATCC 842 at 85%level. Phylogenetic analysis of gene sequence reveals that the gene belonged to β-1,3-1,4-glucanase gene. Alignment using DNAMAN software showed that amino acid sequence of glucanase has active site of glucanase catalyzing β-1,3 and β-1,4-glycosidic bond.
出处
《生物技术通报》
CAS
CSCD
2008年第3期119-122,共4页
Biotechnology Bulletin
基金
山东省优秀中青年科研奖励基金(2006BS06012)
