植酸对胃癌细胞恶性增殖的抑制作用研究

Blocking effect of phytic acid on cell proliferation in human gastric carcinoma

目的研究植酸对bax、bcl-2表达影响与抑制胃癌细胞增殖作用及其机制。方法采用MTT实验法观察植酸对胃癌SGC-7901细胞的增殖抑制作用;倒置显微镜下观察植酸对细胞基本生长状态的改变及形态的变化;单细胞凝胶电泳实验观察植酸对SGC-7901细胞的凋亡诱导作用;Western blotting检测凋亡相关基因bax及bcl-2的蛋白表达。结果MTT实验结果显示,植酸对SGC-7901细胞具有生长抑制作用,并呈现剂量与时间的效应关系;倒置显微镜下的形态学观察表明加药组细胞的生长状态不佳;植酸作用组细胞出现了典型凋亡的彗星施尾,且存在剂量-效应关系;植酸能够下调bcl-2蛋白的表达,植酸处理组细胞中bax蛋白比对照组表达增加,且均呈现剂量-反应关系。结论植酸对SGC-7901细胞具有抑制增殖作用,bax及bcl-2参与了植酸对人胃癌SGC-7901细胞的凋亡诱导作用机制。

Objective To explore the bcl-2 and the bax protein expression, the effect and possible mechanism of phytic acid（IP6）on cell proliferation in human gastric carcinoma. Methods The inhibiting action of IP6 on human gastric carcinoma was examed by MTT assay. The morphological changes of SGC-7901 cells exposed to IP6 was examined by reverse discrepancy microscope. The apoptosis of SGC-7901 cells treated with IP6 was observed by single cell gel electrophoresis. The bax and bcl-2 protein expressions were detected by Western blotting method. Results MTT assay indicated that the growth of SGC-7901 cells were inhibited by IP6 in dose and time dependent manners. The morphological observation by reverse discrepancy microscope indicated that the growth of cells exposed to IP6 were not well. The DNA damage rates of SGC-7901 cells treated with IP6 were more higher than those of control groups in dose and time dependent manners. The bcl-2 protein expressions treated with IP6 were reduced, and the bax protein expressions treated with IP6 were more than those of control groups in dose and time dependent manners. Conclusion The proliferation of gastric carcinoma SGC-7901 cells inhibitited by IP6 could be associated with apoptosis of gene bax and bcl-2.