补充抗氧化维生素对老年机体红细胞功能改善效果的研究

Study the effect of antioxidant vitamin E,vitamin C and β-carotene supplement on erythrocyte functions in elderly person

目的研究抗氧化维生素E（VE）、维生素C（VC）联合不同剂量β-胡萝卜素（β-C）补充，对老年机体红细胞溶血度、红细胞膜ATP酶和红细胞膜流动性的影响。方法经知情同意后选择300名60～75岁的老年人，随机分为5组，1～4组补充VC300mg／d＋VE 200mg／d的同时，再分别补充β-C16．7、8．4、5．6和0．0mg／d，对照5组补充VE 5mg／d，连续补充16周。补充前、后分别抽取清晨空腹静脉血，用H2O2氧化诱导溶血法测红细胞溶血度，酶学比色法测红细胞膜ATP酶活性，荧光偏振法测红细胞膜流动性。结果干预后1～4组老年人红细胞溶血度（分别为38.8％、39．9％、37．6％和40．9％）显著低于其干预前（分别为54．5％、57．1％、56．4％和55．4％）（P〈0．01）和干预后第5组（55．7％）（P〈0．01）；干预后第1组红细胞膜Na^＋ -K^＋ -ATP酶0．72μmol／（mg·h）、Ca^2＋-Mg^2＋-ATP酶0．85μmol／（mg·h）显著高于干预后第5组[分别为0．49μmol／（mg·h）和0．61μmol／（mg·h）]（P〈0，05）；干预后第1～4组红细胞膜荧光偏振度ρ值、1至3组微粘度η值均明显高于其干预前（P〈0．01），也高于干预后第5组（P〈0．01或P〈0．05）。结论抗氧化VE、VC联合不同剂量β-C补充可降低老年机体H2O2诱导的红细胞溶血度，提高红细胞膜ATP酶的活性，改善老年机体红细胞膜的流动性。

Objective To study the effect of antioxidant vitamin E （ VE）, vitamin C （ VC ） combined with different doses of β-carotene （β-C） supplementation on erythrocyte functions in the elderly person. Methods 300 elderly person at the age from 60 to 75years old were randomized into 5 groups： from group 1 to group 4 were supplemented with （VC 300 ＋ VE 200） mg/d and different doses of β-C （16.7, 8.4, 5.6 and 0.0mg/d, respectively）, and control group 5 were supplemented with VE 5mg/d. The trial lasted for 16 weeks. H2O2-caused hemolytic of erythrocytes was measured speetrometriealyl. ATPase activities in erythrocyte membrane were detected by eolorimetrie analysis. The fluidity of erythrocyte membrane was evaluated by fluorescence polarization （ρ） and microviscosity （η）. All the data was analyzed with SPSS12.0. Results H2O2-caused hemolysis of erythrocyte was decreased significantly from group 1 to group 4 after the trial compared with before the trial（ P 〈 0.01 ）, they also had significant difference in compared with group 5 after the trial （ P 〈 0.01 ） . ATPase activities in erythrocyte membrane were more higher in group 1 than those of group 5 after the trial（ P 〈 0.05）. Moreover, ρ and η values were lower from 1 to 4 groups after the trial than itself before the trial （ P 〈 0.01 ）. Conclusion Antioxidant VE, VC combined with different doses of β-C supplementation could improve the function of erythrocyte in the elderly subjects.