茶多酚和表没食子儿茶素没食子酸酯对血管紧张素Ⅱ诱导的心肌肥大的抑制作用

Inhibitory effect of tea polyphenols and EGCG on cultured hypertrophic myocyte induced by AngⅡ

目的体外实验观察茶多酚和表没食子儿茶素没食子酸酯(EGCG)对血管紧张素Ⅱ(AngⅡ)诱导的心肌肥大的抑制作用。方法采用乳鼠心肌细胞原代培养的方法,用AngⅡ处理心肌细胞作为心肌肥大阳性组,观察10、50和100μg/ml的茶多酚和EGCG对AngⅡ诱导的心肌肥大的抑制作用。结果与对照组相比,AngⅡ阳性组心肌细胞总蛋白含量增加、心肌细胞直径增大,而给予不同浓度的茶多酚和EGCG后,与AngⅡ阳性组相比,心肌细胞总蛋白含量和心肌细胞直径则均有一定程度的减少,并呈剂量-效应关系,但细胞数却没有变化。MTT增殖实验显示,AngⅡ可诱导心肌成纤维细胞的增殖,而给予茶多酚和EGCG后对成纤维细胞的增殖则具有一定程度的抑制作用。结论茶多酚和EGCG对AngⅡ诱导的心肌肥大有一定的抑制作用。

Objective To observe the potential effect of tea polyphenols and EGCG on cultured hypertrophic myocytc induced by Ang Ⅱ . Methods The study used cultured neonatal rat myocytes as a model to observe the potential effects of 10μg/ml, 50μg/ml and 100μg/ml tea polyphenols and EGCG on hypertrophic myocyte induced by Ang Ⅱ . We will measure the protein contents, the volume of myocyte, the numbers of fibrocasts. Result When compared to control group （untreated）, the protein contents, the volume of myocytes, the numbers of fibrocasts in Ang Ⅱ group increased. But all thoses in tea polyphenols and EGCG group decreased in a dose-dependent manner, when compared to Ang Ⅱ group. However, the number of the myocyte cells in all groups has no significantly changed. Conclusions Tea polyphenols and EGCG can effectively inhibit hypertrophy of cultured neonatal rat heart myocytes induced by Ang Ⅱ .