油酸型ARDS大鼠心肌组织IL-1β、IL-10含量及mRNA表达的动态观察

Dynamic observatiom of IL-1β,IL-10 and their gene expression in the myocardium of experimental ARDS rats by oleic acid

目的:探讨油酸型急性呼吸窘迫综合征(ARDS)大鼠心肌组织中白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)含量及其mRNA的表达在心肌损伤过程中的作用。方法:Wistar大鼠32只,随机分为4组,采用油酸复制ARDS大鼠模型,观察对照组、油酸(OA)注射后2h(OA2h组)、4h(OA4h组)、6h(OA6h组)组肺的湿/干重、心肌组织的病理改变及血清心肌酶谱(AST、CK、CK-MB、LDH)。用放射免疫分析法检测血清及心肌组织中IL-1β、IL-10的含量,以RT-PCR法检测心肌组织中IL-1β、IL-10mRNA的表达情况。结果:OA各组肺湿/干重均高于C组(P<0.05)。OA各组AST以及OA2h、OA4h组CK、CK-MB、LDH均高于对照组(P<0.05)。OA6h组血清及心肌组织中的IL-1β含量显著升高(P<0.01);OA2h组血清IL-10含量明显高于其它各组(P<0.01),OA6h组心肌组织中IL-10含量明显高于对照组及OA2h组(P<0.01)。OA各组心肌组织IL-1βmRNA表达均高于对照组(P<0.01);OA6h组IL-10mRNA表达有所升高(P<0.01)。结论:油酸型ARDS大鼠并发心肌损伤可能与心肌组织中IL-1β、IL-10含量的增高、mRNA表达的增强有关。

Objective： To study the roles of IL-1β, IL-10 and their gene express in the myocardium of ARDS rats by oleic acid. Methods：Thirty-two Wistar rats were randomly divided into OA experimental groups （OA2h, OA4h, OA6h） and control group. Using radioimmunoassay to measure the levels of IL-1β, IL-10 in serum and homogenate of myocardium. Semi-quantitative RT-PCR is used to measure the IL-1β, IL-10 gene expressions in the myocardium. Serum cardiac enzymes indexes （AST, LDH, CK, CK-MB）, lung W/D, pathological morphology were measured at the same time. Results： In OA6h the level of IL-1β in serum and myocardium were significantly higher than those in control group （P 〈0.01）. Serum levels of IL-10 significantly increased in OA2h group, compared with other groups. IL-10 levels of myocardium significantly increased in OA6h, compared with control and OA2h. The levels of IL-1β gene expression increased in myocardium after OA injection （P 〈0.01）. The expression of IL-10 mRNA in the cardium significantly increased in OA6h. Serum level of CK, CK-MB, LDH in OA2h, OA4h group and AST in OA group were significantly higher than those in control group. Conclusion：The IL-1β, IL-10 contents and its mRNA expressions significantly increased in myocardial tissue of ARDS rat, this maybe lead to heart dysfunction even heart failure.