异丙酚对失血性休克兔肾功能与代谢保护作用

The protection of propofol to metabolism of kidney in hemorrhagic shock New Zealand Rabbit

目的:探讨异丙酚对失血性休克肾功能与代谢的保护作用及其作用的可能机制。方法:建立失血性休克动物模型,选健康新西兰大耳白兔32只,雌雄不拘,随机分为对照组、休克组、灌注组、异丙酚干预组,每组8只。检测血浆尿素氮(BUN)、肌肝(Cr)含量,血浆和肾组织髓过氧化物酶(MPO)活性和丙二醛(MDA)含量变化。结果:休克组和灌注组血浆和肾组织MPO活性增高,与对照组相比差异均有统计学意义(P<0.05～0.01),异丙酚干预组降低,与休克组和灌注组相比差异均有统计学意义(P<0.05～0.01);休克组和灌注组血浆和肾组织MDA含量升高,与对照组和异丙酚干预组比较差异均有统计学意义(P<0.01),异丙酚干预组增高,与休克组相比差异有统计学意义(P<0.01);休克组和灌注组血浆BUN和Cr含量显著升高,与对照组和异丙酚干预组相比差异均有统计学意义(P<0.01)。结论:失血性休克可引起肾组织明显功能代谢障碍,异丙酚干预后各项测定指标的活性、含量均发生变化,异丙酚对失血性休克肾功能与代谢具有一定的保护作用。

Objective： To investijate the propofol pre-treatment effects to thus observed target; Discuss the protection of propofol to hemorrhagic shock＇s nephridium and the mechanism. Methods： A model of hemorrhagic shock New Zealand rabbit was established by imitation. 32 healthy New Zealand rabbits were ran domly divided into 4 groups as following （n =8） ： the control group （CTG）, shock group （group Ⅰ ）, perfusion group （group Ⅱ ） and propofol pre-treatment group （group Ⅲ）. Examine the concentration of blood urea nitrogen （BUN）, creatinine（Cr） in the kidney were measured, and the change of （MPO）, malondialchehyche, （MDA）＇s concentration in the kidney and serum were also measured. Results： The MPO activity in the hemorrhagic shock kidney and serum increased significantly in group Ⅰ and Ⅱ compared with CTG （P 〈0.05-0. 01）. The MDA concentration in the hemorrhagic shock kidney and serum increased in group Ⅰ and Ⅱ compared with CTG and group Ⅲ （P 〈0.01） ; group Ⅱ decreased compared with CTG, group Ⅰ and Ⅲ （P 〈0.05-0.01）. The concentrations of BUN and Cr in serum increased significantly in group Ⅰ and Ⅱ compared with CTG and group Ⅲ （P 〈0.01）. Conclusion： hemorrhagic shock can bring on a significant disfunction of metabolism. From the change of observed target after propofol pre-treatment we can conclude that propofol can take some act of protection metabolism of kidney