新疆维吾尔族原发性高血压与CYP11B2基因-344T/C多态性的关联性

Association of the-344T/C polymorphism of aldosterone synthase gene CYP11 B2 with essential hypertension in Xinjiang uygur isolated group

目的:探讨维吾尔族人群原发性高血压与醛固酮合成酶基因(CYP11B2)-344T/C多态性的关联性。方法:采用聚合酶链反应、限制性片段长度多态性的方法,检测新疆和田地区维吾尔族233例原发性高血压患者(高血压组)和252例正常人群(对照组)醛固酮合成酶基因(CYP11B2)-344T/C多态性。结果:维吾尔族正常人群及高血压患者的-344T/C基因多态性CC、TT、TC基因型频率分布分别为0.167、0.401、0.433和0.155、0.386、0.459,C和T等位基因分布频率分别为0.383、0.617和0.384、0.616,符合Hardy-Weinberg平衡。高血压组与对照组CYP11B2基因-344T/C的C及T等位基因分布差异无统计学意义(χ2=0.001,P=0.97),基因型频率差异无统计学意义(χ2=0.370,P=0.831)。结论:新疆和田地区维吾尔族原发性高血压与CYP11B2基因-344T/C多态性无关联。

Objective： To investigate the association of the -344T/C polymorphism of aldosterone synthase gene CYPllB2 with essential hypertension in Xinjiang Uygur isolated population. Methods： The study covered 233 hypertensive and 252 normotensive controls in Xinjiang Uygur population. The segment of CYPllB2 was amplified from DNA by polymerase chain reaction （PCR）. The PCR products were digested by restriction endonuclease. Result： The frequencies of C and T in hypertensive group （0. 384 and 0. 616） were not significantly different from those in the control group （0. 383 and 0. 617; x^22=0. 001, P =0.97）. The frequencies of-344T/C genotypes of CC, TT and TC were 0. 155, 0. 386and 0. 459 in hypertensive respectively, and 0. 167, 0. 401and 0. 433 in controls respectively. There was no significant difference in genotype between hypertensive group and normotensive group （x^2=0. 370, P =0. 831）. Conclusion： The result suggested that the -344T/C polymorphism of CYPllB2 gene may not be associated with hypertension in Uygur population of Xinjiang.