MGMT基因多态性与食管癌易感性关系

Relationship between MGMT gene polymorphism and susceptibility of esophageal cancer

目的探讨山西省长治地区食管癌患者O6-甲基鸟嘌呤DNA甲基转移酶（MGMT）基因多态性与食管癌易感性的关系,及其与环境因素在食管癌发生中的交互作用。方法采用1∶1配对的病例对照研究,对201对研究对象进行食管癌相关危险因素的问卷调查,应用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术对MGMT多态性基因型进行检测。结果携带MGMT基因杂合型和突变型者发生食管癌的风险增高,χ2=9.80,P〈0.01,OR值分别为1.33（95%CI=1.01～2.07）和1.47（95%CI=1.03～2.54）;MGMT易感基因型与腌制食品对食管癌的发生存在交互作用（S=1.77,API=0.25）,而与吸烟、饮酒、精神刺激、食管癌家族史之间没有交互作用。结论MGMT突变基因型是长治地区食管癌高发的易感基因型,且与腌制食品存在协同作用。

Objective To explore the relationship of polymorphism of O6-methylguanine-DNA-methyltransferase （MGMT） and the susceptibility of esophageal cancer （EC）, and the interaction with environmental factors-gene in Changzhi area. Methods The 1 ： 1 matching case-control study included 201 EC cases and 201 controls. MGMT polymorphisms were determined with polymerase chain-restriction on fragment length polymorphism （PCR-RFLP）. Results The risk of EC significantly increased in individuals with MGMT mutation genotype and heterozygote compared to those with wild genotype （χ^2 =9.80, P〈0.01）. ORs（95% CI） were 1.33（ 1.01 --2.07） and 1.47（1.03--2.54）, respectively. There was interac- tion between MGMT mutation genotype and pickled food （ S = 1.77, API = 0.25）. Conclusion A genetic polymorphism in the MGMT gene may be associated with esophageal cancer in Changzhi area, and there is synergistic action between MGMT predisposing genotype and pickled food.