细胞凋亡的检测方法及其在药物流产中的应用

The Advance of the Examinatorial Method on Apoptosis in Medical Abortion Field

细胞凋亡的检测方法很多,包括细胞凋亡的形态学检测、生化特征检测以及流式细胞仪检测。在药物流产领域中,电镜观察仍是确定凋亡的最具权威的标准,在凋亡定量检测之前应先行电镜观察定性,染色质固缩及凋亡小体形成是其特点。TUNEL技术是目前最常用的凋亡检测技术,在药物流产研究中报道较多,具有较高灵敏性和特异性,可以联合其他方法如免疫荧光技术或流式细胞仪检测等。

There are many examinatorial methods of cellular apoptosis,including morphological detection,detection of biochemical characterization as well as the detection of flow cytometer.In the Field of medical abortion,transmission microscope＇s Observation is the Most authoritative standard to Determine the cellular apoptosis.Before the quantitative detection of apoptosis the qualitative observation by transmission microscope must be first applicated.Its characteristic is the formation of chromatin condensation and apoptotic bodies.Tunel is the most commonly used detection,which is most commonly reported in the study of medical abortion and has high sensitivity and specificity.It can be combined with the other methods such as immunofluorescence technique and flow cytometry.