细胞接触对NK细胞及DC间免疫调节的影响

The Effect of Intercellular Contact on the Immune Regulation between NK Cell and DC

目的探讨自然杀伤细胞(NK)促进树突状细胞(DC)成熟及诱导细胞因子产生的作用,及是否依赖于细胞接触。方法体外培养NK及未成熟DC,并按1∶5、1∶1、5∶1的比例进行接触与非接触式共培养,抗CD86-FITC标记抗体流式细胞术分析DC的CD86表达水平,酶联免疫吸附法检测培养液中TNF-α、IL-12p40含量。结果随着NK/DC比例的升高,表达CD86+DC增多,而DC分泌的TNF-α、IL-12p40呈指数量降低,非接触共培养使DC表达CD86及分泌TNF-α、IL-12p40明显降低。结论NK具有促进DC成熟的作用,并能调节DC细胞因子的分泌,这种免疫调节作用依赖于细胞间接触。

Objective To explore the effects of natural killer cell（NK）on improvement of dendritic cells（DC）maturation and cytokines productions.Methods NK and immature DC cells were cultured in vitro at different ratio,including of intercellular contact and non-contact types.Flow cytometry was used to detect the purity of cells,and enzyme linked immunosorbent assay was used to measure the concentrations of cytokines in culture supernatants.Results With the ratio of NK to DC,the CD86^＋ DC increased and the concentrations of TNF-α and IL-12p40 exponential decreased.Non-contact co-culture led to decreases of CD86 expression and TNF-α,IL-12p40 products.Conclusion It is concluded that NK can induce the maturation and regulate the cytokine productions of DC,which is dependent on intercellular contact.