大鼠同种异体异位节段小肠移植模型的建立

Development of segmental heterotopic small bowel transplantation model in rats

目的:建立大鼠同种异体异位节段小肠移植模型,为研究小肠移植排斥反应提供良好的动物模型。方法:健康成年SD大鼠100只,供、受体各50只,进行同种异体异位节段性小肠移植。结果:预试验10次,术后72h存活率40%。正式实验40次,术后72h存活率85%。全部手术时间平均160min,供体手术时间平均70min,受体手术时间平均90min。结论:良好的血管吻合方法以及输血、补液、保温等围手术期处理是提高手术成功率的关键。

Objective： Establishment of Segmental heterotopic small bowel transplantation （SBT） model in rats in order to study the rejection of SBT. Methods： One hundred healthy adult SD rats, donor and receptor 50 each. Segmental heterotopic SBTs were performed the reconstruction of small intestine. Results： The 72h survival rate of 10 cases in pre-exprimentation was 40% and the 72h survival rate of 40 case undergoing SBT after the model stabilized was 85%. The whole process averagely took 160min. in which the donor manipulation averagely took 70 min and doner manipulation averagely took 90 min. Conclusion： Excellent anastomosis of blood vessels, adequate supplement of blood and liquid, maintenance of body temperature were the keys to success.