重组人粒细胞集落刺激因子对糖尿病大鼠脑缺血后胰岛素样生长因子-1表达的研究

Study on rhG-CSF affecting IGF-1 expression after cerebral ischemia in diabetic rats

目的探讨重组人粒细胞集落刺激因子(rhG-CSF)是否能改善局灶性脑缺血糖尿病大鼠的神经功能,并观察对神经细胞凋亡及胰岛素样生长因子-1(IGF-1)蛋白表达的影响。方法线栓法复制糖尿病大鼠大脑中动脉栓塞模型,rhG-CSF干预组连续皮下注射rhG-CSF,50μg/(kg·d),进行神经功能缺损评分,采用TUNEL染色计数脑缺血周边区神经细胞凋亡数,免疫组织化学方法检测IGF-1的蛋白表达。结果rhG-CSF干预组神经功能缺损评分明显低于对照组(P<0.01);rhG-CSF干预组缺血周边区的TUNEL阳性细胞数均明显少于对照组(P<0.01);IGF-1蛋白免疫阳性细胞明显多于对照组(P<0.01)。结论rhG-CSF可增加糖尿病脑缺血之后缺血周边区IGF-1蛋白的表达,拮抗神经细胞凋亡,改善神经功能。

[Objective] To investigate whether G-CSF could improve neurological deficits, inhibit neuronal cell apoptosis and increase the expression of IGF-1 after cerebral ischemia in diabetic rats. [Methods] Wistar diabetic rats （n =36） were subjected to middle cerebral artery occlusion and randomly divided into two groups： control group （n =18）, G-CSF group （n =18）. G-CSF group received subcutaneous injection of rhG-CSF 50 μg/kg·d after cerebral ischemia, neurological severity scores. TUNEL staining was used to count the number of apoptosis neurons in peri-ischemic area and immunohistochemical assessments was performed to examine the protein expression of IGF-1. [Results] The neurological score significantly increased in G-CSF groups compared with the control group（P 〈0.01）. The expression of IGF-1 significantly increased, apoptosis cells significantly decreased in G-CSF group compared with control group（P 〈0.01）. [Conclusions] RhG-CSF can improve neurological function through inhibition of neuronal cell apoptosis and increasing the expression of IGF-1 after cerebral ischemia in diabetic rats.