眼部淋巴增生病AgNORs的定量及免疫表型研究

Quantitative analysis and immunologi-cal phenotype Study of AgNORs in ocular adnexal lymphoid hyperplasia

目的探讨眼附属器淋巴组织增生性病变的增殖活性与组织学分型的关系．方法用银染色技术对眼部淋巴增生病34例（良性淋巴组织增生14例，不典型淋巴组织增生9例，恶性淋巴瘤11例）进行银染核仁组织区（AgNORS）定量观察。结果发现良性淋巴组织增生与后二者AgNORS颗粒数量间均有极显著性的差异（P＜0．01，P＜0．001），不典型淋巴组织增生与恶性淋巴瘤之间AgNORs颗粒数量相差不显著。结论二种技术对眼附属器淋巴组织增生性病变组织学分型有一定意义。

To investigate the number of AgNORs expression and immunophenotypic lymphocyte analysis in ocularadnexal lymphoid hyperplasia.Methods: Using AgNORs method, the proliferative cellin 34 cases of ocular adnexal pymphoid neoplasms (benignlymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas, ML. 11 cases)were detected in formalin fixed and paraffin-embeded tissues.The immunopheno-type can be served as diagnosis parameterof ocular adnexal lymphoid infiltrations and is of great value indiagnosis of atypical lymphoid hyperplasias.Result: There was a significant difference of AgNORs between BLL and AH, BLL and ML (P＜0.01, P＜0. 001);But there was not a sugnificant difference in AgNORscount between atypical hyperplasia and malignant Iymphoma (P＞0.05).Conclusion: The two methods may be helpful to distinguish ocular adnexal lymphoid reactive hyplasia and malignantlymphoma.