槲皮素诱导人白血病细胞系HL-60细胞程序性死亡

Programmed Cell Death of Leukemic Cell(HL-60) Induced by Quercetin in Vitro

为探讨槲皮素（Quercetin,Que）抑制白血病细胞的作用机制，本文从细胞学、流式细胞术二方面研究Que在体外对人白血病细胞增殖活性的影响．结果表明：Que明显抑制白血病细胞增殖活性，并存在时间和剂量依赖性。其48h的半数抑制浓度（IC50）和95％可信限为43．1（30.5～60.8）umol／L；流式细胞术结果显示Que能诱导HL－60细胞程序性死亡．

To study anti-leukemic cell mechanism of quercetin, its effects on the proliferation activity of human leukemic cell (HL-60) in vitro were investigated by flow cytometric method and MTT assay.The studies indicated that quercetin showed strong suppression on the proliferation activity of leukmic cells in the time and dose-dependent manner. The value of IC50 and 95% confidence limit were 43. 1 (30. 5~60. 8)μmol/L after 48h. The flow cytometry indicated that it could induce programmed cell death of leukmia HL-60 ecll.