复方一枝蒿-壳聚糖纳米药物的制备及其体外抗菌作用的研究(英文)

Preparation and antibacterial activity of compound chitosan-compound Yizhihao-nanoparticles

目的:制备复方一枝蒿-壳聚糖纳米药物并研究其体外抗菌作用。方法:通过乳化交联法合成复方一枝蒿-壳聚糖纳米药物。分别用激光粒度分析仪(Malvern Zetasizer 1000-HAS)和原子力显微镜(AFM)检测该种纳米药物的粒径分布以及表面电位。通过体外抗菌实验检测此种纳米药物的体外抗菌能力,并与传统复方一支蒿颗粒的抗菌能力作比较。结果:该种纳米药物粒径分布均一,为(137.00±14.28)nm;表面携带正电荷,Zeta电位值为(16.90±1.32)mV。其对金黄色葡萄球菌、肺炎球菌、乙型溶血性链球菌和大肠杆菌的最小抑菌浓度分别为1∶32,1∶32,1∶16和1∶2,最小杀菌浓度分别为1∶16,1∶16,1∶8和1∶2。与传统复方一支蒿颗粒相比,该纳米药物对金黄色葡萄球菌、肺炎球菌及乙型溶血性链球菌的抗菌能力均有所改进(P<0.05)。结论:复方一枝蒿-壳聚糖纳米药物对金黄色葡萄球菌、肺炎球菌、乙型溶血性链球菌均具有明显的体外抗菌作用,为我国传统中药新剂型的开发奠定了实验学基础。

Objective To prepare chitosan （ and to investigate its antibacterial activity. Methods CS ） - compound Yizhihao- nanoparticles （ NP ） CS NPs were formed by the incorporation of CS and Nas PO4. CS-compound Yizhihao NPs were prepared by ion-cross-linking. The particle sizes and surface charges of CS NPs were determined by Malvern Zetasizer 1000-HAS and atomic force microscope （ AFM ） , respectively. The antibacterial acitivity of CS-compound Yizhihao-NPs was studied in vitro and compared with that of compound Yizhihao powder. Results Malvern Zetasizer 1000-HAS and AFM demonstrated that the diameter of CS-compound Yizhihao NPs was （137.00± 14.28）nm and CS NPs had （16.90 ± 1.32）mV positive surface charges. The minimal inhibitory concentrations （MIC） of CS-compound Yizhihao NPs on Staphylococcus aureus , Pneumococcus , β- hemolytic streptococcus, and Escherichia coli were 1 ： 32,1 ： 32, 1 ： 16, and 1 ： 2, respectively. The minimal bactericidal concentrations （ MBC ） of CS-compound Yizhihao-NPs on Staphylococcus aureus , Pneumococcus , β-hemolytic streptococcus, and Escherichia coli were 1 ： 16,1 ： 16,1 ： 8, and 1 ： 2, respectively. The antibacterial efficacy of CS-compound Yizhihao-NPs to Staphylococcus aureus, Pneumococcus, and β-hemolytic streptococcus had been improved significantly （P 〈 0.05 ）. Conclusion CS-compound Yizhihao-nanoparticles have obvious antibacterial activity to the Staphylococcus aureus, Pneumococcus, and β-hemolytic streptococcus, which lays the experimental foundation for new preparation of traditional Chinese medicine in future research.