摘要
报道以荧光薄层扫描法(TLCS)测定补益中药中磷脂酰胆碱(PC)的含量。以丙酮和氯仿—甲醇—冰醋酸—乙醇—水(25:4:6:2:0.5),2次同向展开,在碱性硅胶H板上分离PC,经DPH显色后,进行TLCS定量,回收率97.2%,RSD=1.89%,方法简便,灵敏,快速。国内外来见报道。
The fluorescence TLC-scanning method was developed for content determination of phosphatidyl-choline in Chinese materia medicas with tonic action. The method is as follows:being secondarily developed in homo-direction with a mobile phase (acetone-chloroformmethanol-glacial acetic acid-ethyl ester-water(25:4:6:2:0.5),separated on alkaline silicagel-H plate, colorated with DPH,then determed with fluorescence TLC-scanner.As a resultthe recovery is up to 97.2% and RSD is 1.89%. This method is simple, sensitive and quick. No similar experiment report was discovered at home and abroad.
出处
《中成药》
CAS
CSCD
1997年第12期34-35,共2页
Chinese Traditional Patent Medicine
关键词
磷脂酰胆碱
中药
含量
荧光薄层扫描法
flourescence TLC-Scanner,phosphatidyl-choline, Rhizoma Drynariae, Rhizoma Cibotii, Cortex Cinnamomi, Caulis Spatholobi