1[1]Kruger W,Krzizanowski C,Holweg M,et al.Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients.J Cancer Res Clin Oncol,1996,122:679-686.
2[2]Schoenfeld A,luqmani Y,Smith D,et al.Detection of breast cancer micrometases in axillary nodes by using polymerase chain reaction.Cancer Res,1994,54:2986-2990.
3[3]Dile I J,Keafmann M,Costa S D,et al.Micrometastatic breast cancer cell in bone marrow at primary surgery:prognostic value in comparison with nodal status.J Nation Cancer Institute,1996,88:1652.
4[4]Stathopoulou A,Vlachonikolis I,Mavroudis D,et al.Molecular detection of cytokeratin-19-positive cells in the peripheral blood of patients with operable breast cancer:evaluation of their prognostic significance.J clin Oncol,2002,20:3404-3412.
6[6]Borgen E,Beiske K,Trachsel S,et al.Immunocytochemical detection of isolated epithelial cells in bone marrow nonspecific staining and contribution by plasma cells directly reactive to alkaline phosphatase.J Pathol.1998,185:427-434.
7[7]王建中.临床流式细胞分析.上海:上海科学技术出版社,2004:459-490.
8[8]Cruz I,Ciudad J,Cruz J J,et al.Evaluation of multiparameter flow cytometry for detection of breast cancer tumor cells in blood samples.Am J Clin Pathol,2005,123:66-74.
9[9]Kahn H J,Yang L Y,Blondal J,et al.RT-PCR amplification of CK19 mRNA in the blood of breast cancer patients:correlation with established prognostic parameters.Breast Cancer Res Treat,2000,60:143-151.
2Schoenfeld A, Kruger KH, Gomm J, et al. The detection of micrometastases in the peripheral blood and bone marrow of patients with breast cancer using irnmtmohistochemistry and reverse transeriptase polymerase chain reaction for keratin 19. Eur J Cancer, 1997, 33:854-861.
3Finn L, Dougherty G, Finley G, et al. Altematitive splicing of CD44 pre-mRNA in human colorectal tumors. Biochem Biophys Res Commun, 1994,200 : 1015-1022.
4Wielenga VJM, van der Voort R, Muldex JWR, et al. CD44 splice variants as proglaostic markers in colorectal cancer. Scand J Gastroenterol, 1998,33 : 82-87.
5Eltahir EM, Mallinson DS, Bimie GD, et al. Putative markers for the detection of breast carcinoma cells in blood. Br J Cancer, 1998,77 : 1203-1207.
2Revillion F,Hormez L,Peyrat JP.Quantification of c-erbB-2 gene expression in breast cancer by competitive PT-PCR[J].Glin Chem,1997,43(11):2114-2220.
3Felton T,Harris GC,Pinder SE,et al.Identification of carcinoma cells in peripheral blood samples of patients with advanced breast carcinoma using RT-PCR amplification of CK7 and MUG1[J].Breast,2004,13(1):35-41.
4Bostick PJ,Chatterjee S,Chi DD,et al.Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph modes and blood of breast cancer patients[J].J Clin Oncol,1998,16(8):2632-2640.
5Mitas M,Mikhitarian K,Wahers C,et al.Quantitative real-time RT-PGR detection of breast cancer micrometastasis using a multigene marker panel[J].IntJ Cancer,2001,93(2):162-171.
6Bae JW,Ghoi KH,Kim HG,et al.The detection of circulating breast cancer cells in peripheral blood by reverse transcriptase-polymearse chain reaction[J].J Korean Med Sci,2000,15(2):194-198.
7Ghomczynski P,Sacchi N.Single-step method of RNA isolation by add guanidinium thiocyanate-phenol-chloroform extraction[J].Anal Biochem,1987,162(1):156-160.