不同剂量^60Co^γ射线照射正常人淋巴母细胞后的差异表达基因分析

Different gene expression of normal lymphoblastoid cells which exposure to different dose of ^60Co^γ-ray

目的研究不同剂量γ射线照射正常人淋巴母细胞AHH-1的基因差异表达，探讨生物学效应的差异。方法^60Co^γ射线照射AHH-1，用人cDNA芯片检测照射后8h AHH-1细胞和正常细胞mRNA表达，将芯片分析数据进行聚类分析、判别比较，筛选差异表达基因。结果数据分析筛选后，仅与2．0 Gy照射关系密切的差异表达基因23个；仅在0．5 Gy照射中变化的基凶5个；2．0Gy与0．5Gy两组比较变化一致的基因有5个。结论不同剂量γ射线照射AHH-1诱导明显的基因差异表达，其中部分基因表达的改变可能是辐射生物效应发生的关键因素。

Objective To study on the gene expression of normal lymphoblastoid cells（AHH-l） which exposure to difference dose of ^60Co^γ-ray, analyses the essential different biological effect. Methods Human AHH-I normal line was irradiated by ^60Co^γ-rays. Used human cDNA microarray to develop the transcriptional levels of the genes by hybridizing the mRNA of cells 8 h after exposured in different dose and the control cells. Cluster analysis, discrimination and bolting were used to filter the effective genes of differential expression. Results The results of data analysis showed 23 genes of differential expression closely related to biological effect of 2.0 Gy radiation, 5 genes express changed only by 0.5 Gy radiation, 5 genes express apparently both in 2.0 Gy and 0.5 Gy radiation. Conclusion The different dose γ-rays radiation-induced significant changes in gene expression, such as PAPLN, TP53INPI, PTENP1, FOS and TPR seem to be some important components of cellular radioresponse.