EphA2/EphrinA1在胃癌中的表达及其意义

Expression of EphA2 and EphrinA1 and Its Significance in Gastric Carcinoma

目的:探讨EphA2及其配体EphrinA1在胃癌组织中的表达及与胃癌临床病理特征的关系。方法:利用免疫组织化学法检测82例胃癌手术切除标本的癌组织、癌旁组织及正常胃粘膜内EphA2、EphrinA1和E-cadherin的表达;并采用流式细胞术对其中随机选取的45例标本进行上述三种蛋白的定量分析,采用SPSS软件进行统计学处理。结果:胃癌组织中EphA2、EphrinA1的表达明显高于癌旁组织及正常胃粘膜(P<0.01),而癌旁组织与正常胃粘膜之间无显著性差异(P>0.05);E-cadherin在胃癌组织中的表达明显低于癌旁组织和正常胃粘膜(P<0.01),而癌旁组织与正常胃粘膜间无显著性差异(P>0.05)。在胃癌组织中,随分化程度的降低,EphA2、EphrinA1蛋白的表达均显著升高(P<0.05),而E-cadherin蛋白的表达则显著降低(P<0.05);随浸润深度的增加,EphA2、EphrinA1蛋白的表达均显著升高(P<0.05),而E-cadherin蛋白的表达显著降低(P<0.05);淋巴结转移组中EphA2、EphrinA1蛋白的表达显著高于无淋巴结转移组(P<0.05),而E-cadherin蛋白的表达则显著低于无淋巴结转移组(P<0.05);EphA2、EphrinA1和E-cadherin蛋白的表达与肿瘤直径、患者的性别和年龄无关(P>0.05)。EphA2与E-cadherin蛋白的表达呈显著负相关(r=-0.231,P<0.01),EphrinA1与E-cadherin蛋白的表达呈显著负相关(r=-0.403,P<0.01),EphA2与EphrinA1蛋白的表达呈显著正相关(r=0.707,P<0.01)。结论:EphA2/EphrinA1与E-cadherin蛋白表达异常可能共同参与了胃癌的发生、发展与转移;联合检测三种蛋白对于评价胃癌的恶性程度、判断其转移潜能具有一定的参考价值。

Objective： To observe the expression and signifieanee of EphA2 and its ligand EphrinA1 in gastric carcinoma and to investigate the relationship between the expression of these two proteins and clinicopathologie features. Methods： We used immunohistochemistry （IHC） to detect the expression of EphA2. EphrinA1 and E-cadherin in gastric careinoma tissues, adjacent tissues anti normal gastric mucosa from 82 cases of gastric eancer. From the 82 eases, we randomly seleeted 45 cases for detection of the above three proteins by flow cytometry （FCM）. SPSS was employed to analyze the data. Results： The expression of EphA2 anti EphrinA1 was higher in gastric careinoma tissues than in adjacent tissues and nor,hal gastric tissues （P〈0.01, P〈0.01）. E-cadherin expression was lower in gastric carcinoma tissues than in adjacent tissues and normal gastric tissues （P〈0.01, P〈0.01）. No statistieal significance was found in the expression of EphA2, EphrinA1 and E-cadherin between adjacent tissues and normal gastric tissues （P〉0.05, P〉0.05, P〉0.05）. With decreasing histological differentiation, the expression of EphA2 and EphrinA 1 in carcinoma tissues was increased significantly （P〈0.05）, while the E-cadherin expression was reduced significantly （P〈0.05）. With increasing depth of infiltration, the expression of EphA2 and EphrinA1 was enhanced significantly （P〈0.05）, while E-eadherin expression was reduced significantly （P〈0.05）. The expression of EphA2 and EphrinA1 was higher in carcinomas with lymph node metastasis than in those without lymph node metastasis. The expression of these three proteins was not correlated with tumor diameter, age or gender （P〉0.05）. EphA2 expression was negatively correlated with the expression of E-eadherin （r=-0.231, P〈0.01）. A negative eorrelation was also found between the expression of EphrinAl and E-cadherin （r=-0.403, P〈0.01）. The expression of EphA2 waspositively correlated with EphrinAl expression （r=-0.707, P〈0.01）. Conclusion： The abnormal expression of EpbA2, EphrihA1 and E-eadherin may be involved in the carcinogenesis and metastasis of gastric cancer. Detection of these three proteins can provide valuable information for the evaluation of malignant degree and metastatie potential of gastric carcinoma.