温热对HPV感染皮肤中朗格汉斯细胞的影响

Effects of localized hyperthermia on Langerhans cells in HPV-infected skin

目的探讨不同温热条件处理后的正常皮肤，尖锐湿疣皮损组织中朗格汉斯细胞（LC）的形态、数量及迁移。方法利用37℃、42℃、45℃不同的温热条件处理置于培养液中的离体尖锐湿疣皮损（16例）和正常皮肤组织（15例），采用免疫组化和流式细胞分析方法测定LC形态、数量和迁移的变化。结果表皮中LC数量随着温度的增高而减少，正常皮肤组织表皮内LC数量分别为：782．40±114．88（37℃），649．44±119．40（42℃），510．88±118．64（45℃）；疣体组织表皮内LC数量分别为：646．04±135．67（37℃）、489．38±118．19（42℃）、387．93±110．15（45℃）；LC树突变短、减少或消失。温热促进LC迁移至培养液中。不同温度下，游走的LC表达CD1a的数量不同，正常皮肤组织游走至培养液中LC表达CD1a的比率分别是0．19％±0．18％（37℃），0．89％±0．19％（42℃），1159％±0．28％（45℃）；尖锐湿疣组织分别为0．62％±0．31％（37℃），2．31％±0．54％（42℃），6．33％±0．98％（45℃）。结论温热能够促进LC的迁移，籍此有可能增强LC在免疫应答中的抗原提呈作用。

Objective To observe the morphology, quantity and migration of Langerhans cells in normal or HPV-infected skin treated with localized hyperthermia. Methods Tissue specimens obtained from 16 patients with condyloma accuminatum （CA） and 15 normal controls were divided into three equal parts, and irradiated by a self-made hyperthermia equipment at 37 ℃, 42 ℃ and 45 ℃ respectively for 30 minutes. Immunohistochemistry and flow cytometry were applied to detect the morphology, quantity and migration of Langerhans cells （LCs）, respectively, in these treated specimens. Results With a rise in temperature, the number of LCs in epidermis decreased, the dendrites shortened, decreased in number or even disappeared. After exposure to hyperthermia at 37 ℃, 42 ℃ and 45 ℃, the number of LCs was 782.40 ± 114.8, 649.44 ± 119.40 and 510.88 ± 118.64 per square millimeter respectively, in normal tissue, 646.04 ± 135.67, 489.38 ± 118.19 and 387.93 ± 110.15 per square millimeter respectively in HPV-infected skin tissue. The percentage of migratory LCs expressing CDla was 0.19% ± 0.18%, 0.89% ± 0.19% and 1.59% ± 0.28% in normal skin tissue treated with hyperthermia at 37 ℃, 42 ℃ and 45 ℃ respectively, 0.62% ± 0.31%, 2.31% ± 0.54% and 6.33% ± 0.98%, respectively, in HPV-infected skin tissue; the differences were significant among these different temperatures. Furthermore, the migration of LCs from tissue into culture was enhanced by the treatment with hyperthermia. Conclusions Hyperthermia can promote the migration of LCs, and accordingly enhance the antigen presenting effect of these cells in immune response.