凋亡抑制蛋白c—FLIP在银屑病患者外周血和皮损中的表达

Expression of cellular FLICE-inhibitory protein in peripheral blood and lesions of patients with psoriasis

目的探讨凋亡抑制蛋白c—FLIP在寻常性银屑病患者外周血和皮损中的表达和分布情况。方法采用流式细胞仪检测30例寻常性银屑病患者和20例正常人对照组外周血T细胞和B细胞内c—FLIP蛋白表达阳性率，采用免疫组化方法检测c—FLIP蛋白在其皮损中的表达。结果进行期银屑病患者外周血T细胞内c—FLIP表达（6．32％±1．17％）明显高于恢复期患者（2．64％±0．74％，P〈0．01）和正常人对照组（2．28％±0．54％，P〈0．05）。而其在外周血B细胞内的表达3组间差异无统计学意义，3组分别为0．78％±0．16％，0．71％±0．32％，0．69％±0．18％，P值均〉0．05。c—FLIP蛋白在进行期银屑病患者皮损中的表达（89．73±5．24）明显高于恢复期（117．40±7．50，P〈0．05）和正常人对照组（121.58±7．93，P〈0．01），恢复期患者和正常人对照组之间差异无统计学意义（P〉0．05）。结论凋亡抑制蛋白c—FLIP在进行期银屑病患者的外周血T细胞和皮损内明显高表达，可能参与银屑病患者T细胞的增殖。

Objective To investigate the expression and distribution of cellular FLICE-inhibimry protein （c-FLIP） in peripheral blood and lesions of psoriatic patients. Methods Peripheral blood and skin samples were obtained from 30 patients with psoriasis vulgaris and 20 normal consols. Flow cytometry was used to detect intracellular c-FLIP protein in peripheral T and B lymphocytes, immunohistochemistry to examine the expression of c-FLIP in lesional tissue. Results Based on the positivity rate of c-FLIP, there was a significant increase in T lymphocytes in active psoriasis compared with regressive psoriasis and normal controls （6.32% ± 1.17% vs 2.64% ± 0.74% and 2.28% ± 0.54%, P〈 0.01 and 0.05, respectively）, while no significant difference was found in B lymphocytes among these three groups （0.78% ± 0.16%, 0.71% ± 0.32%, 0.69% ± 0.18%, respectively, P 〉 0.05 ）. The expression intensity of c-FLIP in keratinocytes was also higher in active psoriasis than in regressive psoriasis and normal controls （89.73 ± 5.24 vs 117.40 ± 7.50, 121.58 ± 7.93, P 〈 0.01 and 0.05 respectively）, and there was no difference between regressive psoriasis and normal controls （P 〉 0.05 ）. Conclusions c-FLIP is highly expressed in lesions and peripheral T lymphocytes of patients with active psoriasis, suggesting the possible involvement of c-FLIP in the proliferation of T lymphocytes in psoriasis.