他克莫司对人角质形成细胞增殖及干细胞因子分泌的影响

Effects of tacrolimus on cell proliferation of and secretion of stem cell factors by cultured human keratinocytes

目的探讨他克莫司对人角质形成细胞的增殖及干细胞因子（SCF）分泌的影响。方法体外HaCaT细胞（人永生化角质形成细胞）培养，用MTY和ELISA分别测定他克莫司对HaCaT细胞增殖及SCF含量的影响。结果10^3nmol／L～10^4nmol／L的他克莫司对人HaCaT细胞增殖有抑制作用。10nmol／L～10^2nmol／L的他克莫司可以促进HaCaT细胞SCF分泌量的增加；10^3nmol／L的他克莫司对HaCaT细胞SCF的分泌无影响；高浓度104nmol／L的他克莫司则可抑制HacaT细胞SCF的分泌。结论适当浓度的他克莫司能够促进角质形成细胞SCF的分泌。

Objective To study the effects of tacrolimus on the cell proliferation of and secretion of stem cell factors （SCF） by cultured human kcratinocytcs. Methods Human kcratinocytc cell line HaCaT was cultured and treated with various concentrations （0, 10, 10^2, 10^3, 10^4 nmol/L） of tacrolimus. After 48 hours of treatment, cell proliferation was measured with MTT assay , the levels of stem cell factor in the culture supcrnatant of HaCaT cells were detected with ELISA. Results Inhibited proliferation was observed in HaCaT cells treated with tacrolimus of 10^3-10^4 nmol/L. The secretion of SCF by HaCaT cells was enhanced in the presence of 10-10^2 nmol/L of tacrolimus, was inhibited in the presence of tacrolimus of 10^4 nmol/L, and remained unchanged with 10^3 nmol/L. Conclusion Certain concentrations of tacrolimus could enhance the expression of SCF in HaCaT cells, which may be associated with the efficacy of tacrolimus in the treatment of vitiligo.