期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

伊钮小单孢菌2-脱氧蟹肌醇氨基转移酶基因sisM的克隆与表达

Cloning and expression of 2-deoxy-scyllo-inosose aminotransferase gene sisM from Micromonospora inyoensis
下载PDF
导出
摘要 从伊钮小单孢菌(Micromonospora inyoensis)中扩增到参与西索米星生物合成基因sisM,其开放阅读框长1263bp,编码含421个氨基酸残基(44.3ku)的蛋白质。经Blast比对,该蛋白与已报道的L-谷氨酰胺:2-脱氧蟹肌醇(DOI)氨基转移酶有很高的同源性,据此推测sisM是编码DOI氨基转移酶的基因。该基因在IPTG诱导下.在大肠埃希菌BL21(DE3)实现了表达,表达产物的分子量与推测的一致。这是从伊钮小单孢菌克隆到的又一参与西索米星核心基团2-脱氧链霉胺(DOS)生物合成的新基因,该基因在GenBank的登录号为EU074791。 The gene sisM, a novel gene involved in sisomicin biosynthesis was cloned from Micromonospora inyoensis by PCR. Its ORF is 1,263 bp coding 421 amino acids （44.3ku）. A Gapped-BLAST search showed that the deduced sisM gene product is highly homologous to the previously reported L-gluatamine. 2- deoxy-scyllo-inosose aminotransferase. Based on protein homologies, the product of sisM is suggested as a L- gluatamine.2-deoxy-scyllo-inosose aminotransferase. Then it was over-expressed by IPTG induction in E. coli BL21 （DE3）. The molecular weight observed in the SDS-PAGE was similar to that calculated from amino acid sequences of sisM. sisM is another DOS biosynthetic gene involved in sisomicin biosynthesis in M. inyoensis. Its accession number in GenBank is EU074791.
作者 曾志红 洪文荣 罗敏玉 戈梅 陈代杰
机构地区 福州大学生物科学与工程学院 上海来益生物药物研发中心 上海医药工业研究院
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2008年第5期269-272,共4页 Chinese Journal of Antibiotics
关键词 sisM L-谷氨酰胺:2-脱氧蟹肌醇氨基转移酶 西索米星 伊钮小单孢菌 sisM L-gluatamine. 2-deoxy-scyllo-inosose aminotransferase Sisomicin Micromonospora inyoensis
分类号 R978.19 [医药卫生—药品] R978 [医药卫生—药品]
  • 相关文献

参考文献17

  • 1Dworkin R J. Aminoglycosides for the treatment of gramnegative infections: therapeutic use, resistance and future outlook[J]. Drug Resist Updat,1999,2(3):173-179.
  • 2Livermore D M. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? [J]. Clin Infect Dis,2002,34(5) : 634-640.
  • 3Zembower T R, Noskin G A, Postelnick M J, et al. The utility of aminoglycosides in an era of emerging drug resistance [J]. Int J Antimicrob Agents,1998,10(2):95-105.
  • 4Litovchick A, Lapidot A, Eisenstein M, et al. Neomycin B-arginine conjugate, a novel HIV-1 Tat antagonist: synthesis and anti-HIV activities [J]. Biochemistry, 2001,40 (51) :15612-15623.
  • 5Kawaguchi H, Naito T, Nakagawa S, et al. BB-K 8, a new semisynthetic aminoglycoside antibiotic [J]. J Anti -biot ,1972 ,25(12 ) :695-708.
  • 6Nagabhushan T L, Cooper A 13, Tsai H, et al. The syntheses and biological properties of 1-N-(S-4-amino-2- hydroxybutyryl )-gentamiein B and 1-N-( S-3-amino-2- hydroxypropionyl)-gentamicin B [J]. J Antibiot, 1978,31 (7) :681-687.
  • 7Miller G H, Arcieri G, Weinstein M J, et al. Biological activity of netilmicin, a broad-spectrum semisynthetic aminoglycoside antibiotic [J]. Antimicrob Agents Chemother,1976,10(5) :827-836.
  • 8Kharel M K, Basnet D B, Lee H C, et al. Molecular cloning and characterization of a 2-deoxystreptamine biosynthetic gene cluster in gentamicin-producing Micromonospora echinospora ATCC15835 [J]. Mol Cells, 2004,18(1):71-78.
  • 9Kharel M K, Subba B, Basnet D B, et al. A gene cluster for biosynthesis of kanamycin from Streptomyces kanarnyceticus: comparison with gentamicin biosynthetic gene cluster[J]. Arch Biochem Biophy, 2004,429 (2) : 204-214.
  • 10Kharel M K, Basnet D B, Lee H C, et al. Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptornyces tenebrarius[J]. FEMS Microbiol Lett,2004,230(2):185-190.

二级参考文献13

  • 1Unwin J, Standage S, Alexander D, et al. Gene cluster in Micromonospora echinospora ATCC15835 for the biosynthesis of the gentamicin C complex [J]. J Antibiot, 2004,57(7):436-445.
  • 2Nakano M M, Mashiko H, Ogawara H. Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species [J]. J Bacteriol, 1984,157(1): 79-83.
  • 3Ohnuki T, Imanaka T, Aiba S. Self-cloning in Streptomyces griseus of an str gene cluster for streptomycin biosynthesis and streptomycin resistance [J]. J Bacteriol, 1985,164 (1):85-94.
  • 4Kojic M, Topisirovic L, Vasiljevic B. Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis [J]. J Bacteriol , 1992,174(23):7868-7872.
  • 5Kojic M, Topisirovic L, Vasiljevic B. Translational autoregulation of the sgm gene from Micromonospora zionensis [J]. J Bacteriol,1996,178(18):5493-5498.
  • 6Kharel M K, Basnet D B, Lee H C, et al. Molecular cloning and characterization of a 2-deoxystreptarnine biosynthetic gene cluster in gentarnicin-producing Micromonospora echinospora ATCC15835 [J]. Mol Cells,2004,18(1):71-78.
  • 7GenBank Database. MPU575934, M. purpurea DOS biosynthesis gene cluster, http://www, ncbi. nlm. nih.gov, 2005-07-20.
  • 8Kharel M K, Subba B, Basnet D B, et al. A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: comparison with gentamicin biosynthetic gene cluster [J]. Arch Biochem Biophys, 2004,429(2):204-214.
  • 9Kharel M K, Basnet D B, Lee H C, et al. Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius [J]. FEMS Microbiol Lett , 2004,230(2):185-190.
  • 10Kharel M K, Subba B, Lee H C, et al. Characterization of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius [J]. Bioorg Med Chem Lett, 2005,15 (1): 89-92.

共引文献4

中国抗生素杂志
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部