大鼠视神经切断后视网膜双极细胞PKC-α和recoverin的表达

Expression of PKC-α and recoverin in rat retinal bipolar cells after the optic nerve transection

为了探讨视神经切断后视网膜内部是否存在突触可塑性改变,本实验采用大鼠视神经切断模型,通过免疫组织化学方法检测视神经切断后视网膜双极细胞PKC-α和recoverin的表达变化。结果显示:正常视网膜中,PKC-α和recoverin阳性产物主要见于视网膜内核层、内网层及节细胞层,另外外核层也可见少量recoverin阳性细胞。视神经切断后3d,大鼠视网膜内网层高倍镜下可见PKC-α和recoverin免疫阳性终末的数量开始增加,14d时增至最高,21d、28d呈现逐渐减少的趋势。本研究结果提示视神经切断后视网膜双极细胞与节细胞之间的突触可能存在早期增生,后期溃变的可塑性变化。

To discuss whether there exists synapse plasticity in the retina after optic nerve injury, the optic nerve transection （ONT） model was used in this study and the expressional change of PKC-α and recoverin in the retinal bipolar cells was detected by the immunohistochemistry. The results showed that PKC-α and recoverin positive products in normal retina were mainly localized in the inner nuclear layer （INL） , inner plexiform layer（IPL） and ganglion cell layer （GCL）, and recoverin positive products also can be observed in the outer nuclear layer （ONL）. Compared with the normal control, the number of PKC-α and recoverin positive terminal in the IPL gradually increased at 3 days after the optic nerve transection and reached to peak at day 14, then dropped at day 21 and 28. This data suggest that after optic nerve transection, there exist synapse plasticity which is increased early and decreased later in the retinal bipolar cells and retinal ganglion cells （RGCs） .