大鼠受损视神经中神经前体细胞的变化及预变性腓总神经的调节

Changes of neural precursor cells in the injured optic nerve of rat and effect of common peroneal nerve autograft

为了研究大鼠受损视神经内神经前体细胞的变化及调节,本研究建立了成年雄性大鼠视神经损伤及自体腓总神经移植模型,分正常组、损伤组和移植组,体外培养视神经的神经前体细胞,在相差显微镜下观测各组神经前体细胞神经球的形态和数目,以免疫荧光细胞化学方法对神经球细胞进行鉴定。结果显示:正常组神经球较小、较少,多数细胞表达nestin、GFAP或半乳糖脑苷脂(GC);视神经损伤后神经球的总数有所增加,但大神经球数明显减少,nestin、GFAP或GC阳性细胞也明显减少;神经移植后增加了各类神经球数,nestin、GFAP或GC阳性细胞也明显增加。本研究提示成年大鼠视神经内神经前体细胞较少,增殖能力较弱;视神经损伤也伤及其神经前体细胞并抑制其增殖;自体神经移植能保护神经前体细胞并促进其增殖。

In order to study the changes and regulation of neural precursor cells in the injured optic nerve of rat, an optic nerve injury model and an autogeneic common peroneal nerve transplantation model were made. Animals were divided into 3 groups ： normal, injury and transplantation groups, Neural precursor cells from the optic nerve were cultured in vitro. Morphology and number of the neurospheres formed by neural precursor cells of optic nerve in different groups were measured under a phase contrast microscope, Cells were identified by immunofluorescent cytochemical staining, The results showed that less and smaller neurospheres were found in normal group. Most of the cells were nestin-, GFAP- or GC- immunoreactivity in the neurosphere. The total number of neurospheres somewhat increased after optic nerve injury, but the big neurospheres and the nestin-, GFAP- or GC- positive cells decreased obviously, The number of different kinds of neurospheres increased largely after common peroneal nerve transplantation, the nestin-, GFAP- or GC-positive cells obviously increased too. All results suggest that there are less neural precursor cells with weak proliferation in adult rat optic nerve. Neural precursor cells are also injured after the optic nerve injury and its proliferation is depressed. Nerve autograft may protect the neural precursor cells and facilitate its proliferation.