青岛沿海九种海藻的类脂及酚类抗菌活性的研究

ANTIMICROBIAL ACTIVILIES OF SEVERAL SEAWEEDS IN QINGDAO COAST

利用双碟法和两种有针对性的提取方法:先用索氏提取器乙醚可流13h提取类脂然后用80％乙醇提取酚类,研定了青岛沿海常见的9种海藻的抗菌能力。结果表明,9种海藻的所有提取物对枯草杆菌都有明显抑制作用,只有3种提取物对大肠杆菌有明显的抑制作用。一些用甲苯:甲醇(1:3)提取筛选证明无抑菌能力的海藻也显示具有明显的抑制效果。此外,利用气相色谱法测定了其中6种脂类中脂肪酸的相对含量,但不饱和脂肪酸的合量与抗菌能力无正相关性。

Duplicate plates methods and two purposive extraction methods (extracting phenols with 80% ethanol for 3 times after extracting lipids with other for 13 hours in soxhlot extractor)were used to research antimicrobial ability of nine common seaweeds in Qingdao coast. All extracts of nine seaweeds showed considerable activity against Bacillus subtilis, however,only three extracts,belonging to three species respectively,were effective against Escherichia coli. Several seaweeds not showing antimicrobial activity in previous tests showed considerabal effects. Gas chromatography was applied to analyse the contents of fatty acids in six kinds of lipids. C12-C22 saturated and unsaturated fatty acids were detected but no positive relation between the contents of unsaturated fatty acids and inhibition abilities were found.