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罗格列酮对高糖诱导的人肾小管上皮细胞血小板反应蛋白1和BMP-7表达的影响 被引量：2

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摘要 目的:探讨罗格列酮(RSG)对高糖环境下人肾小管上皮细胞(HK-2)血小板反应蛋白1(TSP-1)和骨形成蛋白7(BMP-7)mRNA和蛋白表达的影响。方法:将体外培养的人肾小管上皮细胞分为正常糖对照组(NG)、高糖组(HG)、渗透浓度对照组(MG)、分别含RSG5、10、20μmol/L的高糖DMEM培养基培养的RSG干预组(R5、R10、R20),并于培养48h后终止培养。免疫细胞化学方法检测人肾小管上皮细胞TSP-1、BMP-7、结缔组织生长因子(CTGF)蛋白的表达,RT-PCR法检测人肾小管上皮细胞中TSP-1、BMP-7、CTGFmRNA表达。结果:高糖环境下,HK-2细胞TSP-1、CTGFmRNA和蛋白表达明显升高(P<0.05),BMP-7mRNA和蛋白表达明显降低(P<0.05),加入RSG后,HK-2细胞TSP-1、CTGF的表达较NG组明显降低(P<0.05),BMP-7的表达明显升高(P<0.05)。结论:RSG可以抑制高糖诱导的人肾小管上皮细胞TSP-1、CTGF高表达,提高BMP-7的表达,具有一定的肾脏保护作用。
作者 丁新国 李英 李晓东 张红梅
机构地区 河北医科大学第三医院肾内科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2008年第6期639-641,共3页 Chinese Journal of Cellular and Molecular Immunology
关键词 血小板反应蛋白-1 骨形成蛋白-7 罗格列酮 高糖 肾小管上皮细胞
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献10

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共引文献7

同被引文献16

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引证文献2

二级引证文献3

细胞与分子免疫学杂志
2008年 第6期

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