靛蓝及其同类色素的微生物生产与转化

Microbial Biosynthesis and Biotransformation of Indigo and Indigo-like Pigments

靛蓝类色素广泛应用于印染、食品和医药工业,其环境友好的合成或生产途径越来越受到人们的关注,特别是微生物生物合成。已经鉴定和分离了能够合成靛蓝类色素的多种微生物,并且明确了起催化作用的主要是单加氧酶和双加氧酶。已经克隆和利用了一些加氧酶的基因,构建了工程菌,优化了其发酵过程。同时,微生物合成靛蓝的生物转化也已经起步。这些进展将带来环境友好的靛蓝类色素的合成与生产。

Indigo and indigo-like pigments are widely used in the industry of textile, food and medicine. Now people pays more and more attention to developing an alternative method of indigo production which is ＂environment-friendy＂, especially microbial biosynthesis of indigo. Many microorganisms involved in the biosynthesis of indigo have been isolated and characterized, and monooxygenase and dioxygenase have been identified to catalyze indigo biosynthesis. Some genes encoding for these enzymes have been cloned and used to construct ＂engineering bacteria＂. With this kind of bacteria, more efficient fermentation systems for indigo production have been exploited. In the meantime, biotransformation of the indigo produced by microorganisms has been under investigation. These progresses will bring us a greener method of indigo and indigo-like pigments production.